HPLC同时测定蜘蛛香中9种指标成分的含量

Simultaneous Determination of Nine Components in Valeriana jatamansi Jones by HPLC

目的:建立高效液相色谱法(HPLC)同时测定蜘蛛香中新绿原酸、绿原酸、咖啡酸、异绿原酸B、橙皮苷、异绿原酸A、异绿原酸C、乙酰缬草三酯及缬草三酯含量的方法。方法:采用DiamonSil C 18色谱柱,乙腈(A)-0.1%甲酸水溶液(B)为流动相梯度洗脱,检测波长为327 nm(0~33 min)、256 nm(33~90 min),流速为1.0 mL/min,柱温为30℃。结果:新绿原酸在0.75~120.00 mg/L(r=1.0000)、绿原酸在5.23~418.00 mg/L(r=1.0000)、咖啡酸在0.44~44.00 mg/L(r=1.0000)、异绿原酸B在2.1~210.00 mg/L(r=1.0000)、橙皮苷在2.20~220.00 mg/L(r=1.0000)、异绿原酸A在2.065~330.000 mg/L(r=0.9999)、异绿原酸C在1.88~150.00 mg/L(r=1.0000)、乙酰缬草三酯在2.50~200.00 mg/L(r=1.0000)、缬草三酯在10.00~800.00 mg/L(r=1.0000)的进样浓度范围内线性关系良好,加样回收率(n=6)为101.92%~107.51%,RSD<3%;25批蜘蛛香样品中9种成分含量差异较大,其中缬草三酯、橙皮苷、异绿原酸A及绿原酸等4个成分的含量较高。结论:HPLC可同时测定蜘蛛香中9种成分含量。

Objective:To establish an HPLC method for the simultaneous determination of nine constituents in Valeriana jatamansi,including neochlorogenic acid,chlorogenic acid,caffeic acid,hesperidin,isochlorogenic acid B,isochlorogenic acid A,isochlorogenic acid C,acevaltrate and valepotriate.Methods:The analysis of this drug was performed on a DiamonSil C 18 column,with acetonitrile(A)-0.1%formic acid(B)solution as the mobile phase for gradient elution,the detection wavelength at 327 nm(0~33 min)and 256 nm(33~90 min),the flow rate at 1.0 mL/min,and the column temperature at 30℃.Results:Good linear relationships of neochlorogenic acid,chlorogenic acid,caffeic acid,hesperidin,isochlorogenic acid B,isochlorogenic acid A,isochlorogenic acid C,acevaltrate and valepotriate was showed within the ranges of 0.75~120.00 mg/L(r=1.0000),5.23~418.00 mg/L(r=1.0000),0.44~44.00 mg/L(r=1.0000),2.10~210.00 mg/L(r=1.0000),2.20~220.00 mg/L(r=1.0000),2.065~330.000 mg/L(r=0.9999),1.88~150.00 mg/L(r=1.0000),2.50~200.00 mg/L(r=1.0000),10.00~800.00 mg/L(r=1.0000)respectively;the average recoverie rates(n=6)were between 101.92%~107.51%and each RSD was under 3%;the contents of nine components in 25 batches of Valeriana Jatamansi Jones were different,and the contents of valepotriate,hesperidin,isochlorogenic acid A and chlorogenic acid were the highest.Conclusion:HPLC can be used for simultaneous determination of 9 components in Valeriana Jatamansi.