养阴益气合剂对肺纤维化大鼠TGF-β/Smad信号通路的影响

Effects of Yangyin Yiqi mixture on TGF-β/Smad signal pathway of bleomycin-induced pulmonary fibrosis rats

目的研究养阴益气合剂对博来霉素诱导的肺间质纤维化大鼠TGF-β/Smad信号通路的影响。方法将120只SPF级Wistar大鼠随机分为空白组、模型组、泼尼松组及养阴益气合剂低、中、高剂量组,采用气管滴注博来霉素的方法复制肺纤维化大鼠模型,造模后第二天各组予相应药物灌胃,连续给药28天。分别于第14天和28天分批处死大鼠,留取肺组织以实时荧光定量PCR法检测转化生长因子-β1(transforming growth factor-β1,TGF-β1)、Smad3及Smad7表达,留取血清以酶联免疫法检测羟脯氨酸(Hydroxyproline,HYP)表达。结果与空白组相比,模型组TGF-β1、Smad3 mRNA及HYP表达明显升高,Smad7显著减少(P<0.05)。治疗后,肺纤方中、高剂量组TGF-β1、Smad3及HYP出现显著下降,Smad7表达上调(P<0.05)。结论养阴益气合剂的抗肺纤维化作用可能与抑制TGF-β/Smad通路,减少细胞外基质沉积有关。

Objective To investigate the effects of Yangyin Yiqi mixture on transforming growth factor-beta/Smad signal pathway of bleomycin-induced pulmonary fibrosis rats.Methods 120 Wistar rats were randomly divided into 6 groups:normal control group,model group,prednisone group,low-dose,mid-dose,and high-dose Yangyin Yiqi mixture groups.Bleomycin was used to establish the pulmonary fibrosis model rats.From the second day after modeling,each group was given a corresponding drug gavage for 28 days.The rats were killed on the 14th and 28th day respectively.The lung tissues were collected to detective the mRNA expression of transforming growth factor-beta1,Smad3 and Smad7 by realtime-PCR method.Serum was collected to detective the expression of hydroxyproline by Elisa method.Results Compared with the normal group,the expression of TGF-β1,Smad3 and HYP in the model group significantly increased,and Smad7 significantly decreased(P<0.05).After treatment,the expression of TGF-β1,Smad3 and HYP in the middle and high dose groups of Yangyin Yiqi mixture decreased significantly,and Smad7 expression was up-regulated(P<0.05).Conclusion The anti-pulmonary fibrosis effect of Yangyin Yiqi mixture may be related to inhibiting TGF-β/Smad pathway and reducing extracellular matrix deposition.