紧密连接蛋白Claudin-1、7在日光性角化病和皮肤鳞状细胞癌中的表达

Expression of Claudin-1 and Claudin-7 in actinic keratosis and squamocellular carcinoma

目的:检测紧密连接蛋白Claudin-1、7在日光性角化病(AK)和皮肤鳞状细胞癌(SCC)中的表达水平。方法:收集2014年11月至2016年11月我院病理科AK和SCC组织标本各30例,选取癌旁正常皮肤组织30例作为对照组,采用免疫组化方法检测癌旁正常表皮、AK和SCC组织中Claudin-1、7蛋白的表达。结果:对照组、AK和SCC组织中Claudin-1蛋白细胞阳性率分别为100%,53.33%和13.33%,三组两两比较,差异均有统计学意义(P s<0.05)。Claudin-7在癌旁正常表皮、AK和SCC组织中均呈阴性表达。结论:Claudin-1蛋白在癌旁正常表皮、AK和SCC组织中表达水平逐渐下降,Claudin-1蛋白表达下调可能与表皮肿瘤的恶性转化有关。

Objective:To detect the expression level of Claudin-1 and Claudin-7 in the lesions of actinic keratosis(AK)and squamocellular carcinoma(SCC).Methods:The expression levels of Claudin-1 and Claudin-7 were detected by immunohistochemical method in the 30 normal tissues adjacent to cancer,30 AK lesions,30 SCC lesons in our hospital from November,2014 to November,2016.Results:In the samples of normal controls,AK and SCC,the positivity rates of Claudin-1 cells were 100%,53.33%and 13.33%respectively,with significant differences among three groups(P s<0.05).Claudin-7 was negatively expressed in normal skin tissues,AK and SCC.Conclusion:The expression level of Claudin-1 protein decreased gradually in the adjacent normal skin tissues,AK and SCC tissues,and the down-regulation of Claudin-1 protein expression may be related to the malignant transformation of epidermal tumors.