槲皮黄酮通过Sphk2抑制人皮肤鳞状细胞癌A431细胞的增殖

Quercetin inhibits the proliferation of human skin squamous cell carcinoma A431 cells through sphingosine kinase 2

目的:研究槲皮黄酮(Quercetin,Qu)对人皮肤鳞状细胞癌A431细胞增殖活性的影响。方法:采用CCK8法、Western blot实验和流式细胞术检测不同浓度下(10、50、100、200μg/mL)A431细胞活力、增殖及凋亡相关指标;Western blot检测Qu对A431细胞中Sphk2信号通路的影响。结果:10、50、100、200μg/mL Qu作用96 h后,细胞OD值分别为(0.654±0.098)、(0.527±0.089)、(0.412±0.076)、(0.309±0.054);细胞活力分别为(81.3±4.6)%、(56.3±4.9)%、(37.4±4.2)%、(19.4±3.6)%;细胞凋亡率分别为(6.43±1.09)%、(14.77±1.26)%、(21.30±1.36)%、(29.84±1.26)%。Qu呈浓度依赖性的抑制细胞中Sphk2蛋白表达,且Qu+Sphk2 mimics组细胞活力明显高于于Qu,细胞凋亡率明显低于Qu组(P s<0.05)。结论:Qu可能通过抑制Sphk2信号通路降低人皮肤鳞状细胞癌细胞的增殖活性。

Objective:To study the effects of quercetin(Qu)on the proliferative activity of human skin squamous cell carcinoma cells(A431).Methods:The viability,proliferation and apoptosis of A431 cells treated by QU of different concentration(10,50,100 and 200μg/mL)were respectively detected by CCK-8 assay,Western blot and flow cytometry assay.The impacts of Qu on activation of sphingosine kinase 2(Sphk2)in A431 cells were detected by Western blot.Results:After treated for 96h by QU of concentration 10,50,100 and 200μg/mL,the OD readings were(0.654±0.098),(0.527±0.089),(0.412±0.076)and(0.309±0.054),respectively;the cell activities were(81.3±4.6)%,(56.3±4.9)%,(37.4±4.2)%,(19.4±3.6)%,respectively and the apoptosis rate of A431 cells were(6.43±1.09)%,(14.77±1.26)%,(21.30±1.36)%and(29.84±1.26)%,respectively.Qu dose-dependently inhibited the expression levels of Sphk2 in A431 cells,and the cell activity of A431 cells in Qu+Sphk2 mimics was higher and the apoptosis rate was lower than that in Qu group(P s<0.05).Conclusion:Qu could inhibit the proliferative activity of human skin squamous cell carcinoma cells through decreasing the activation of Sphk2 pathway.