C57BL/6J、129/SvJ及其杂交一代小鼠的行为学比较研究

Comparative Studies on Behavior of C57BL/6J、129/SvJ and Their Hybrid Mice

目的比较C57BL/6J和129/SvJ两种不同遗传背景及其杂交一代(F1)在行为学表型上的差异。方法将C57BL/6J小鼠和129/SvJ小鼠交配繁育,得到两种F1小鼠129B6F1和B6129F1,连同C57BL/6J和129/SvJ按照不同性别分为8组,每组小鼠数量n≥12,进行旷场活动、悬尾、水迷宫、被动回避、热板实验等行为学研究,分别从焦虑、抑郁、学习记忆、热痛四个方面比较C57BL/6J和129/SvJ小鼠及F1小鼠的行为表现。结果在旷场活动中,与129/SvJ比较,F1在外周、边缘的活动差异显著(P<0.01);在悬尾活动中,F1的行为没有显著性差异;在水迷宫和被动回避活动中,与C57BL/6J比较,F1寻找到平台的潜伏期和进入暗箱的潜伏期差异显著(P<0.05)。结论 F1的不同行为表型受到亲代的不同遗传背景影响。

Objective To compare the genetic backgrounds of C57BL/6 J and 129/SvJ and the behavioral phenotypes with their hybrid(F1) mice. Methods C57BL/6 J and 129/SvJ were cross mated and bred to obtain two F1(129 B6 F1 and B6129 F1). These F1 mice with C57BL/6 J and 129/SvJ were divided into 8 groups of experimental mice according to different gender. Numbers of mice in each group were no less than 12. Behavioral studies such as the open field test, the tail suspension test, the morris water maze, the passive avoidance test, the hot plate were conducted to compare the behavioral performance of C57BL/6 J, 129/SvJ and F1 from the paramters of anxiety, depression,learning and memory, and heat pain response respectively. Results Compared with 129/SvJ, F1 had significant difference in peripheral and marginal activities in the open field activities(P<0.01).There was no significant difference in the behavior of F1 and parent mice in the tail suspension activities. Compared with C57BL/6 J, the latency of finding the platform and entering the dark box of F1 were significantly different in the morris water maze and the passive avoidance activities(P<0.05).Conclusion Different behavior phenotypes of F1 are influenced by different genetic backgrounds of their parents.