HPLC法同时测定化妆品中补骨脂素、异补骨脂素、新补骨脂异黄酮和补骨脂二氢黄酮含量

HPLC determination of psoralen, lsopsoralen, neobavaisoflavone and bavachin in cosmetics

采用高效液相色谱法研究了化妆品中补骨脂素、异补骨脂素、新补骨脂异黄酮和补骨脂二氢黄酮的含量测定,得到了补骨脂素、异补骨脂素、新补骨脂异黄酮和补骨脂二氢黄酮分别在0.1091~21.8144 mg/L、0.1067~21.3328 mg/L、0.1086~21.7128 mg/L、0.1123~22.4644 mg/L范围内线性关系良好,平均回收率为94.78%~104.74%,RSD均小于5%。

An analytical method for the simultaneous determination of psoralen,lsopsoralen,neobavaisoflavone and bavachin in cosmetics by HPLC were developed.The linear relationships are good in the ranges of 0.1091-21.8144 mg/L,0.1067-21.3328 mg/L,0.1086-21.7128 mg/L and 0.1123-22.4644 mg/L for the four components,respectively.The average recoveries are 94.78%-104.74%with the RSD less than 5%.