无角牦牛FTO基因多态性与生长性状的关联性分析

Correlation between FTO gene polymorphism and growth traits of polled yak

【目的】探究无角牦牛脂肪和肥胖相关基因(FTO)多态性与生长性状之间的关联性,以期找到与无角牦牛生长相关的分子标记。【方法】参考GenBank中野牦牛的FTO基因序列,针对其第4内含子和第8内含子设计引物,采用PCR-SSCP和DNA测序技术对354头无角牦牛的FTO基因进行多态性分析,并运用SPSS 20.0软件分析各基因与体质量、体高、体斜长和胸围等生长性状的相关性。【结果】在无角牦牛FTO基因的第4内含子区域检测出A164506C突变位点,有2个等位基因A和C,有3种基因型(AA、AC和CC),优势等位基因和基因型分别是A和AA,其频率分别为0.6949和0.4859;在第8内含子区域检测出G309000A突变位点,共有2个等位基因A和G,有3种基因型(GG、GA和AA),优势等位基因和基因型分别是G和GG,其频率分别为0.9263和0.8584。A164506C突变位点为中度多态位点,纯合度较低,处于Hardy-Weinberg平衡状态;G309000A突变位点为低度多态位点,纯合度较高,处于Hardy-Weinberg不平衡状态。A164506C位点与无角牦牛的体高和体斜长具有显著或极显著相关性,AC基因型个体优于AA基因型个体。G309000A位点与无角牦牛的胸围和体斜长具有显著或极显著相关性,GA基因型个体优于GG基因型个体。【结论】FTO基因第4内含子区域A164506C突变和第8内含子区域G309000A突变均与无角牦牛生长性状具有相关性。

【Objective】This study explored the association between fat and obesity-related gene(FTO)polymorphisms and growth traits of polled yak,aiming to find molecular markers related to the growth of polled yak.【Method】The FTO gene sequence of wild yak in GenBank was used to design primers for the 4th intron and the 8th intron.PCR-SSCP and DNA sequencing were used to analyze the FTO gene of 354 polled yak,and SPSS 20.0 was used to analyze the correlation between each gene and growth traits including body mass,body height,body length and chest circumference.【Result】The A164506C mutation site was detected in the 4th intron region of the FTO gene with two alleles A and C and three genotypes(AA,AC,and CC).The dominant allele and genotype were A and AA with frequencies of 0.6949 and 0.4859,respectively.In the 8th intron region,there were 2 alleles A and G detected in the G309000A mutation site and 3 genotypes(GG,GA and AA).The dominant allele and genotype were G and GG with frequencies of 0.9263 and 0.8584,respectively.The A164506C mutation site was a moderate polymorphic locus and in the Hardy-Weinberg equilibrium state with low homozygosity.The G309000A mutation site was a low polymorphic locus and in Hardy-Weinberg imbalance with high homozygosity.The A164506C locus had significant or extremely significant correlation with height and body length of polled yak.The AC genotype was superior to the AA genotype.The G309000A locus had significant or extremely significant correlation with chest circumference and body length of polled yak.The GA genotype individuals were superior to the GG genotype individuals.【Conclusion】The A164506C mutation in the 4th intron region of the FTO gene and the G309000A mutation in the 8th intron region were associated with polled yak growth traits.