滁菊的功能成分及其体外抗氧化活性

Functional Components and in Vitro Antioxidant Activity of Chrysanthemum Flower (Chrysanthemum morifolium Ramat) from Chuzhou

为明确滁菊中抗氧化活性物质的含量及其抗氧化能力,对其进行进一步的开发利用,对滁菊(Chrysanthemum morifolium Ramat)酚类、多糖、类胡萝卜素提取物抗氧化能力及含量进行研究。结果表明:滁菊总酚和黄酮含量较高,分别为53.67 mg/g和70.32 mg/g,高于大洋菊等菊花;多糖和类胡萝卜素含量较低。3种提取物中,酚类抗氧化能力最强,对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)和2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)(2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)阳离子自由基的清除能力分别为208.79 mmol/g和160.23 mmol/g,对Fe3+的还原能力达到238.35 mmol/g,高于杭白菊等菊花。采用高效液相色谱法测定了滁菊酚类及类胡萝卜素单体组成,初步鉴定出滁菊含有绿原酸、儿茶素、咖啡酸、阿魏酸、金丝桃苷、槲皮素6种单体酚,以及叶黄素和β-类胡萝卜素2种类胡萝卜素单体。酚类物质中绿原酸含量最高,达到813.21 mg/g;金丝桃苷次之,达到408.17 mg/g;类胡萝卜素中叶黄素含量最高,为78.6 mg/g。在不考虑协同作用情况下,酚类物质中绿原酸对Fe3+还原能力、DPPH及ABTS阳离子自由基清除能力的贡献均最大,类胡萝卜素中叶黄素对DPPH自由基的清除能力贡献最大。

The antioxidant composition and activity of chrysanthemum flowers from Chuzhou,Anhui were determined for its further exploitation and utilization.The contents of phenolics,carotenoids and polysaccharides in the extracts of chrysanthemum flowers were measured and their antioxidant activities were investigated in this study.The total phenolic and total flavonoid contents in chrysanthemum flowers from Chuzhou were 53.67 and 70.32 mg/g,which were higher than those in Dayang chrysanthemum flowers,while the contents of polysaccharides and carotenoids were relatively lower.Among the three extracts,the phenolic extract was presented the strongest antioxidant activity with scavenging capacity of 208.79 mmol/g against 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical and of 160.23 mmol/g against 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)cation radical as well as ferric reducing antioxidant power(FRAP)value of 238.35 mmol/g.By high performance liquid chromatography(HPLC),six phenolic acids such as catechin,caffeic acid,chlorogenic acid,ferulic acid,hyperin and quercetin were identified as well as two carotenoids such as lutein andβ-caroteonid.The content of chlorogenic acid was the highest(813.21 mg/g)of these phenolic compounds,followed by hyperin(408.17 mg/g).Lutein was the most abundant carotenoid(78.6 mg/g).Chlorogenic acid made the greatest contribution to the ferric reducing power,while hyperin made the greatest contribution to the DPPH and ABTS cation radical scavenging activities when not considering synergism.Lutein in carotenoids contributes most to the of DPPH free radical scavenging activity.