姜黄素对幽门螺杆菌及其诱导人胃GES-1细胞损伤的影响

Effect of Curcumin on Helicobacter pylori and GES-1 Cells Injured by It

对姜黄素抑制幽门螺杆菌(Helicobacter pylori,Hp)及其诱导人胃GES-1细胞损伤的影响进行研究。抑菌实验结果显示,姜黄素可明显抑制Hp生长,其最低抑制浓度为200μmol/L。采用Berthelot显色法检测Hp脲酶活力的变化情况,发现姜黄素对其也有明显的抑制作用,半数抑制浓度为1.735 mmol/L。通过噻唑蓝实验分析姜黄素对人胃GES-1细胞和Hp感染人胃GES-1细胞的影响。结果显示,短时间内(12 h)较低浓度(68μmol/L)姜黄素对人胃GES-1细胞增殖无明显影响,但姜黄素浓度的增加和作用时间的延长可使细胞存活率明显下降。经68μmol/L姜黄素作用12 h后,损伤模型组细胞形态有一定程度的复原,细胞存活率略有升高,但不显著(P>0.05);高浓度(136、680μmol/L)姜黄素会导致细胞存活率明显下降。因此,姜黄素对Hp生长和脲酶活力有明显的抑制作用,但不足以缓解Hp对人胃GES-1细胞的损伤作用。

The inhibition of curcumin on Helicobacter pylori(Hp)and its effect on GES-1 cell injury induced by Hp were investigated.The bacteriostatic tests showed that curcumin significantly inhibited Hp proliferation with a minimum inhibitory concentration(MIC)of 200μmol/L.Moreover,curcumin showed an obvious inhibitory effect on the urease activity of Hp with a half maximal inhibitory concentration(IC50)of 1.735 mmol/L as determined using jack bean urease model based on Berthelot reaction.The effect of curcumin on normal and Hp-infected GES-l cells was studied by using the MTT method.Curcumin could inhibit human gastric epithelial GES-1 cells in a dose-and time-dependent manner except for treatment for 12 h at a concentration of 68μmol/L.After treatment with curcumin at 68μmol/L for 12 h,the morphology of the cell injury model,infected with Hp for 24 h,was slightly restored,and the survival rate slightly increased(P>0.05).But the survival rate significantly decreased after treatment with curcumin at 136 or 680μmol/L.Thus,the inhibition of curcumin on the viability and urease activity of Hp is not sufficient to help repair Hp-induced damage in GES-1 cells.