支气管哮喘患儿血浆颗粒蛋白前体表达水平及其与氧化应激、哮喘控制效果的关系

Plasma progranulin expression in children with bronchial asthma and its relationship with oxidative stress and asthma control effect

目的研究支气管哮喘患儿血浆颗粒蛋白前体表达水平及其与氧化应激、哮喘控制效果的关系。方法选取2017年2月至2018年8月该院收治的支气管哮喘患儿140例作为观察对象,其中包括急性期组75例与缓解期组65例,另取同期于该院接受体检的健康儿童60例作为健康对照组。分别采用酶联免疫吸附法检测3组人员的血浆颗粒蛋白前体水平,采用水溶性四唑盐法、比色法与微板法检测丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)水平并做相关性分析。结果急性期组、缓解期组、健康对照组人员血浆颗粒蛋白前体表达水平分别呈逐渐降低趋势,且经单因素方差分析可得:差异有统计学意义(P<0.05)。急性期组、缓解期组、健康对照组人员的MDA水平呈逐渐降低趋势;急性期组、缓解期组、健康对照组SOD、GSH水平呈逐渐升高趋势,差异有统计学意义(均P<0.05)。经Pearson相关性分析显示,支气管哮喘患儿血浆颗粒蛋白前体表达水平与MDA呈正相关(r=0.477,P=0.011),与SOD、GSH水平呈负相关(r=-0.523、-0.581,P=0.000、0.000)。结论支气管哮喘患儿血浆颗粒蛋白前体表达水平与哮喘控制效果、氧化应激存在密切相关,即随着血浆颗粒蛋白前体表达水平的不断升高,哮喘控制效果越差,且氧化应激反应越明显。

Objective To study investigate the relationship between plasma progranulin expression and oxidative stress and asthma control effect in children with bronchial asthma.Methods 140 cases of children with bronchial asthma admitted to a hospital from February 2017 to August 2018 were recorded as objects of observation,including 75 cases in the acute stage group and 65 cases in the remission stage group.Another 60 cases of healthy children who received physical examination in a hospital during the same period were selected as the normal control group.Levels of plasma progranulin in the three groups were detected by enzyme-linked immunosorbent assay,and the levels of MDA,SOD and GSH were detected by WST,colorimetry and microplate assay,and their correlation was analyzed.Results The levels of plasma progranulin in acute,remission,and normal control groups were showing a gradually decreasing trend,and the results of one-way anova showed that there were statistically significant differences among the groups(all P<0.05).The MDA levels in acute,remission and control groups decreased gradually.The levels of SOD and GSH in the acute stage group,the remission stage group and the normal control group showed a gradually increasing trend,the differences between the groups were statistically significant(all P<0.05).Pearson correlation analysis showed that the expression level of plasma progranulin in children with bronchial asthma was positively correlated with MDA(r=0.477,P=0.011),and negatively correlated with SOD and GSH levels(r=-0.523,-0.581,P=0.000,0.000).Conclusion The expression level of plasma progranulin in children with bronchial asthma is closely related to the control effect of asthma and oxidative stress,that is,with the increasing expression level of plasma progranulin,the worse the control effect of asthma is,and the more obvious the oxidative stress response is.