miR-144-5p、miR-668-3p、miR-134-5p在儿童原发性免疫性血小板减少症中的表达

The expression of miR-144-5p, miR-668-3p and miR-134-5p in children with primary immune thrombocytopenia

目的:探讨外周血单个核细胞(PBMCs)中mi R-144-5p、mi R-668-3p、mi R-134-5p在儿童原发性免疫性血小板减少症(ITP)中的表达变化。方法:采用RT-q PCR检测25例ITP患儿和15例正常对照PBMCs中3种mi RNAs相对表达量,Sysmex XE-5000全自动血细胞分析仪检测血小板计数及未成熟血小板分数(IPF%);分析mi RNAs与血小板计数的相关性,ROC曲线分析检验效能。结果:ITP患儿组PBMCs中的mi R-144-5p和mi R-668-3p较对照组表达均上调,mi R-134-5p表达下调(均P<0.05)。mi R-144-5p和mi R-668-3p在急性ITP患儿组中表达量要高于慢性ITP患儿组(均P<0.05),且miR-144-5P(r=-0.802,P<0.001)和miR-668-3p(r=-0.753,P<0.001)的相对表达量与外周血小板计数呈负相关。相较于单个指标,miR-144-5p联合IPF%诊断急性ITP的AUC为0.960,灵敏度和特异度分别为93.3%和100%;而mi R-134-5p联合IPF%诊断慢性ITP的AUC为0.967,灵敏度和特异度分别为90.0%和93.3%。结论:mi R-144-5p和mi R-668-3p在ITP患儿PBMCs中表达显著上调,mi R-134-5p表达下调,这些改变可能在疾病的发生与发展中发挥了重要作用。

Objective: To investigate the changes of microRNAs(miR-144-5 p, miR-668-3 p and miR-134-5 p) in peripheral blood mononuclear cells(PBMCs) in children with primary immune thrombocytopenia(ITP). Methods: Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR) was used to detect the relative expression of the three miRNAs from 25 ITP patients and healthy control in PBMCs. Platelet count and immature platelet fraction(IPF%) were detected by Sysmex XE-5000 automatic blood analyzer. The correlations of the miRNAs expression with peripheral platelet count were analyzed and diagnostic value was measured by receiver operating characteristic curve. Results: Compared with the normal control group, the expressions of miR-144-5 p and miR-668-3 p of the ITP group in PBMCs were significantly up-regulated(P<0.05), and were negatively correlated with platelet count(r=-0.802, P<0.001;r=-0.753, P<0.001), while the expressions of miR-134-5 p was down-regulated significantly(P<0.05). The expressions of miR-144-5 p and miR-668-3 p in the acute ITP group were higher than those in the chronic ITP group(P<0.05). In terms of the single indicator, the AUC of miR-144-5 p combined with IPF% in the diagnosis of acute ITP was 0.960. The sensitivity and specificity were 93.3% and 100% respectively. The AUC of miR-134-5 p combined with IPF% in the diagnosis of chronic ITP was 0.967. The sensitivity and specificity were 90.0% and 93.3% respectively. Conclusion: miR-144-5 p and miR-668-3 p in PBMCs were significantly up-regulated and miR-134-5 p was down-regulated in children with ITP, which suggests they may play an important role in the incidence and development of ITP.