摘要
目的:观察程序性死亡配体-1(PD-L1)在结直肠癌组织及癌旁组织中的表达,探索其与患者临床病理特征之间的相关性,并探讨PD-L1表达水平在结直肠癌中的临床意义。方法:选取2016年1月至2017年1月温州医科大学附属第一医院50例结直肠癌患者,手术中获取癌组织及癌旁组织,采用PCR及Western blot检测结直肠癌及癌旁组织中PD-L1 mRNA及蛋白水平,并分析其与临床病理特征之间的相关性。结果:与癌旁组织相比,结直肠癌组织中PD-L1基因及蛋白表达量均显著升高(P<0.05)。癌组织中PD-L1蛋白阳性率与癌组织分化程度和淋巴转移相关(P<0.05)。结论:PD-L1在结直肠癌组织中表达增高,并与结直肠癌患者的临床病理特征密切相关。
Objective:To investigate the expression of PD-L1 in colorectal cancer and its relationship with clinicopathologic features,and meanwhile to discuss the clinical significance of PD-L1 expression.Methods:Colorectal cancer tissues and adjacent normal tissues were obtained from 50 patients with colorectal cancer.RTPCR and Western blot were conducted to detect the expression of PD-L1 in mRNA and protein levels.Results:The expression of PD-L1 in colorectal cancer tissues was elevated,compared with that in adjacent normal tissues(P<0.05).The elevated expression of PD-L1 in colorectal cancer tissues was correlated with cancer differentiation degree and lymphatic metastasis(P<0.05).Conclusion:PD-L1 was highly expressed in colorectal cancer patients,which was correlated with the clinicopathologic features.
作者
杜舟
黄萍
黄国裕
韩少良
左志贵
DU Zhou;HUANG Ping;HUANG Guoyu;HAN Shaoliang;ZUO Zhigui(Department of Hernia and Abdominal Wall Surgery,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325015,China;Office of Development and Planning,Wenzhou Medical University,Wenzhou 325035,China;Department of Gastrointestinal Surgery,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325015,China;Department of Colorectal Anal Surgery,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325015,China)
出处
《温州医科大学学报》
CAS
2020年第1期41-44,共4页
Journal of Wenzhou Medical University
基金
温州市公益性科技计划项目（2017Y1031）
关键词
结直肠癌
程序性死亡配体-1
癌旁组织
免疫逃逸
colorectal cancer
programmed deathligand 1
adjacent normal tissue
immune response
