辐射事故受照人员药物转运体基因表达的变化

Changes of drug transporter gene expression in radiation accidental exposed persons

研究电离辐射引起的迟发性药物转运体基因表达的变化。以3例受60Coγ射线照射3 a后的患者外周血为研究对象,采用Agilent全基因组基因表达芯片技术,筛选出药物转运体差异表达基因。对溶质转运体差异基因表达谱研究发现,3名事故受照人员有机离子转运体和糖类转运体的基因表达以上调为主,氨基酸/胺类转运体和无机离子类转运体基因表达上调或下调趋势不明显,其它类转运体的基因表达则以下调表达为主;对ATP结合盒(ABC)转运体差异基因表达谱研究发现,ABC转运体基因表达以参与多药耐药的转运体基因表达变化为主。结果表明,3名受照人员的多个药物转运体基因表达均发生了改变,其中,一些临床药物是这些药物转运体的底物,可能会改变这些药物的药代动力学特征,为电离辐射对药物的吸收、分布和排泄影响的研究提供参考。

To study the changes in delayed drug transporter gene expression induced by ionizing radiation,the differential expression of drug transporter genes in the peripheral blood of three people exposed accidentally to 60Co γ-rays during 3a after irradiation were analyzed using the Agilent whole genome gene expression microarray assay.The differentially expressed solute transporters(SLC)genes were studied.It was found that the genes of organic ion transporters and carbohydrate transporters were mainly up-regulated,while those of the other transporters were mainly down-regulated.There was no obvious up regulation and down regulation in the expression of amino acid/amine transporters and inorganic ion transporter genes.The differentially expressed genes of ATP-binding cassette transporter(ABC)found that ABC transporter genes expression was mainly focused on multi-drug resistance transporter genes.The gene expression profiles of multiple drug transporters were altered in the three people who were accidentally exposed to radiation.Some clinical drugs that they were administered are substrates of these drug transporters,may change the pharmacokinetic profiles of these drugs.These data also provided a reference for studying the effects of ionizing radiation on drug absorption,distribution,and excretion.