纳米金刚石对癫痫细胞模型SOD、GSH-Px、MDA的影响

Effect of Nano-Diamonds on SOD, GSH-PX, MDA of Epileptic Cell Model

目的探讨纳米金刚石对癫痫细胞模型SOD、GSH-Px、MDA的影响。方法取新生24 h内SD乳鼠海马神经元进行原代培养,培养9天后随机分为正常对照组、癫痫组、纳米金刚石组。用无镁细胞外液建立癫痫细胞模型,电镜下观察细胞形态数目变化,CCK8试剂盒检测细胞活性,并检测细胞内和培养液丙二醛(MDA)、超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH-Px)的含量。结果与正常对照组比较,癫痫组的细胞活性下降,细胞内SOD、GSH-Px含量明显降低,MDA含量明显升高,培养液中SOD活性降低,但MDA含量未升高;加入纳米金刚石后,细胞活性升高,细胞内SOD、GSH-Px含量恢复正常水平,MDA含量降低,培养液中SOD活性增加,但MDA含量无明显变化。结论纳米金刚石可以通过抗氧化应激作用减轻无镁致痫海马神经元的损伤。

Objective To investigate the effect of nano-diamonds on SOD, GSH-PX, MDA of epileptic cell model. Methods Primary neuronal cultures were prepared from the hippocampus of SD newborn rats within the first 24 h. After 9 days of culture, they were randomly divided into normal control group, epilepsy group and nano-diamonds group. The epileptic cell model was established with magnesium-free extracellular fluid. Changes in both cell morphology and number were observed by electron microscopy and cell viability was detected by CCK8 kit. The intracellular and culture solution malondialdehyde(MDA), superoxide dismutase(SOD), as well as prototype glutathione(GSH-Px) contents were detected. Results Compared with normal control group, the cell viability of epilepsy group decreased, also, the contents of SOD and GSH-Px in cells decreased significantly while the MDA content increased significantly. In culture solution, the activity of SOD decreased, but the MDA content did not increase. After adding nano-diamonds, the cell viability increased. Besides, the contents of both SOD and GSH-Px in cells returned to normal while the MDA content decreased. In culture solution, the activity of SOD increased,however, its MDA content had no obvious change. Conclusions Nano-diamonds is capable of alleviating the damage of hippocampal neurons caused by the absence of magnesium through anti-oxidative stress.