珍珠龙胆石斑鱼(Epinephelus fuscoguttatus♀×E.lanceolatus♂)幼鱼溃疡病病原分离及鉴定分析

Isolation and identification of pathogen from juvenile pearl gentian grouper(Epinephelus fuscoguttatus♀×E.lanceolatus♂) with skin ulcer disease

本研究对患溃疡病的珍珠龙胆石斑鱼(Epinephelus fuscoguttatus♀×E.lanceolatus♂)幼鱼进行病原分离和鉴定。从患病鱼分离到一株优势菌HW2018,回归感染实验证实其为致病菌。经形态学观察、16S rDNA以及生理生化等综合鉴定,菌株HW2018为弧菌属哈维氏弧菌(Vibrio harveyi)。3×10^4 cfu/g肌肉和腹腔注射,周死亡率均达到100。药敏分析结果显示,哈维弧菌HW2018对多种抗生素敏感,对碳青霉烯类的美罗培南、氟喹诺酮类的环丙沙星和左氧氟沙星尤为敏感;对氨苄西林耐药。

A bacterial strain,named HW2018,was isolated from juvenile pearl gentian grouper(Epinephelus fuscoguttatus♀×E.lanceolatus♂)with skin ulcer disease and identified as Vibrio harveyi by morphological observation,physiological and biochemical characteristics and phylogenetic analysis of 16S rDNA sequences.Artificial infection experiment proved that the strain HW2018 was the causative pathogen.Death rate in one week was 100%^with the injection dose(3×10^4 cfu/g)through celiac injection or muscle injection.The drug sensitivity test revealed that V.harveyi was sensitivity to meropenem,ciprofloxacin and levofloxacin,but resistant to ampicillin.