紫玉盘茎中低极性化学成分的气相色谱-质谱联用分析

GC-MS analysis of the low polarity components in the stems of Uvaria macrophylla Roxb. var. microcarpa(Champ.)Finet et Gagnep.

目的研究紫玉盘茎中主要的低极性化学成分。方法紫玉盘茎用95%乙醇80℃加热回流提取,提取液蒸发浓缩除去乙醇后,用石油醚萃取,得到石油醚提取物,运用GC-MS法对其主要成分进行检测,所得结果与标准谱库进行匹配分析,用峰面积归一法计算各成分的相对含量。结果鉴定出10个化学成分,相对含量较高的有十六烷酸(16.76%)、4(14)-11-桉油烯(15.31%)、蛇床烯(14.45%)。结论检测出紫玉盘茎石油醚萃取部位的化学成分以萘类芳香烃化合物(31%)为主,倍半萜类化合物(29.76%)次之。

Objective To investigate the low polarity components in the stems of Uvaria macrophylla Roxb.var.microcarpa(Champ.)Finet et Gagnep.(Annonaceae).Methods The dry stems of the title plant were extracted by refluxing with 95%ethylalcohol(80℃),and the obtained aqueous alcohol solution was evaporated to remove alcohol,and then the ethyl alcohol extracts were extracted with petroleum ether(PE)to obtain a PE extract.The chemical constituents in the PE extract were analyzed by GC-MS and identified by comparison of the obtained MS data with the standard mass spectral data.The relative contents in percentage were calculated for the constituents using the area normalization method.Results Ten compounds were identified,in which the palmitic acid(16.76%),4(14)-11-eucalypene(15.31%),and selinene(14.45%)were the main components.Conclusion The low polarity components in the stems of Uvaria macrophylla Roxb.var.microcarpa(Champ.)Finet et Gagnep.mainly consisted of naphthalenes(31%),followed by sesquiterpenoids(29.76%).