调环酸钙在马铃薯及植株中的残留和消解分析

Residue and Dissipation Analysis on Prohexadione-calcium in Potato and Plant

调环酸钙作为植物生长调节剂广泛用于禾谷科作物、马铃薯及其他作物中,其在农作物中的残留引起了人们的广泛关注。研究比较了4种不同提取溶剂对调环酸钙的提取效率以及2种分散固相萃取净化剂对提取液的净化效果,最终确立了马铃薯和马铃薯植株样品用酸化乙腈振荡提取,盐析后取有机相经超高效液相色谱-串联质谱测定的残留检测方法。调环酸钙在马铃薯中的平均回收率为87.8%~99.0%,相对标准偏差为1.7%~3.2%;在马铃薯植株中的平均回收率为93.9%~98.0%,相对标准偏差为2.1%~4.8%。调环酸钙在马铃薯中的最小检测浓度为0.02 mg/kg。采用田间试验方法研究了调环酸钙在马铃薯植株中的消解动态规律,半衰期为1.4~1.5 d;在马铃薯样品中的残留量均<0.02 mg/kg。

Prohexadione-calcium as a plant growth regulator has been widely used in cereal crops, potatoes and other crops, and its residues in crops have been taken more and more attentions. After the extraction efficiencies of four different extraction solvents for prohexadione-calcium and the purification effects of two dispersive solid-phase extraction purifiers on the extract were compared, a method of determination prohexadione-calcium in potatoes and potato plants was developed. Samples were extracted with acidic acetonitrile, and the supernatant organic phase was determined by UPLC-MS/MS after salting and concentration. The average recoveries of prohexadione-calcium in potatoes were 87.8%-99.0%, with their relative standard deviations were 1.7%-3.2%. The average recoveries of prohexadione-calcium in potato plants were 93.9%-98.0%, with their relative standard deviations were 2.1%-4.8%. The minimum detectable concentration of prohexadione-calcium in potatoes was 0.02 mg/kg. The dissipation dynamic rhythm of prohexadionecalcium in potato plants was investigated in a field experiment, and the half-lives(DT50) were 1.4-1.5 d. The final residues of prohexadione-calcium in potatoes were <0.02 mg/kg.