1株来源于茅台酒酿造过程宛氏拟青霉MTDF-01的全基因组测序及分析

Whole-Genome Sequencing and Analysis of Paecilomyces variotii MTDF-01, Isolated during Maotai-Flavor Liquor Brewing

为深入了解宛氏拟青霉(Paecilomyces variotii)在酱香型白酒生产过程中代谢机理,为今后进一步挖掘及调控P. variotii在白酒酿造过程中代谢提供必要生物信息学基础。通过PacBio RS II测序平台对P. variotii MTDF-01进行全基因组测序,并且对测序数据进行基因组组装、基因预测和功能注释、碳水化合物基因预测、次级代谢产物合成基因簇预测,以及与已报道全基因组序列的分离于土壤中的耐甲醛P. variotii No.5进行比较基因组分析和共线性分析。基因组拼接得到19 个基因组骨架和19 个重叠群,总长度为30 833 540 bp,GC平均含量为47.46%。基因组中预测到8 815 个基因、5 044 个简单重复序列,221 个tRNA,49 个rRNA。总共注释基因8 662 个,其中淀粉和纤维素水解酶基因分别有60 个和165 个,次级代谢产物合成基因簇23 个。P. variotii MTDF-01与P. variotii No.5基因组存在翻转、异位等基因组重排,两者基因组共存在16 414 处单核苷酸碱基突变,其中有525 个碱基缺失,335 个碱基插入,15 554 个单碱基替换。本研究利用PacBio RS II测序平台获得了P. variotii MTDF-01的全基因组信息,并且分析P. variotii MTDF-01的潜在功能,为深入了解P. variotii在白酒生产过程中的代谢机理提供了遗传信息基础,对今后酱香型白酒中风味和健康物质的调控研究具有重要意义。

This study aimed to gain insight into the metabolic mechanism of Paecilomyces variotii in the production of Maotai-flavor liquor for the purpose of providing an essential bioinformatic basis for further understanding and regulating the metabolism of this strain. The whole-genome sequencing of P. variotii MTDF-01, a strain isolated during the brewing of Maotai-flavor liquor, was carried out on the PacBio RS II platform. The sequencing reads were assembled and annotated via gene prediction and functional annotation. In the whole genome, 19 scaffolds and 19 contigs were obtained. The total length was 30 833 540 bp and the average GC content was 47.46%. A total of 8 815 genes, 5 044 simple sequence repeats (SSR), 221 tRNA and 49 rRNAs were predicted. The number of annotated genes was 8 662. The genome contained 60 starch hydrolase-coding genes and 165 cellulose hydrolase-coding genes, as well as 23 secondary metabolite biosynthetic gene clusters. The genome rearrangement of MTDF-01 was different from that of P. variotii No.5, a formaldehyde-resistant strain isolated from soil. Synteny analysis indicated that genome rearrangement and translocation occurred among these genomes. A total of 16 414 single nucleotide base mutations were found in the genomes of the two strains, including 525 base deletions, 335 base insertions and 15 554 single base substitutions. The high-quality whole-genome sequence and potential function of P. variotii MTDF-01 obtained in this study are of great significance for future studies on the regulation of the flavor and health-benefiting substances of Maotai-flavor liquor.