miR-21表达变化与结直肠癌肿瘤发生发展的关系

Relationship between expression of miR-21 and tumorigenesis and progression of colorectal cancer

[目的]探讨结直肠癌组织中的微小RNA21(miR-21)表达与肿瘤发生发展的关系。[方法]选取我院2015年1月~2016年9月确诊的结直肠癌组织标本及其对应的癌旁组织标本各80例,采用实时荧光定量聚合酶链反应(qRT-PCR)技术检测2组的miR-21水平,并分析不同TNM分期、病理学分化程度、病理学类型、肿瘤直径、肿瘤位置、淋巴结转移结直肠癌组中的miR-21表达差异。[结果]结直肠癌组织中的miR-21表达强度显著高于癌旁组织,且差异有统计学意义(P<0.05);不同病理学类型、不同分化程度、不同病灶直径的结直肠癌组织中的miR-21表达强度组间比较,差异无统计学意义;在TNM分期(Ⅲ期+Ⅳ期)、发生淋巴结转移、不同预后结局的结肠癌组织中的miR-21表达强度显著地高于TNM分期(Ⅰ期+Ⅱ期)、未发生淋巴结转移的结直肠癌组织,差异有统计学意义(P<0.05)。结直肠癌患者TNM分期增高、发生淋巴结转移、miR-21表达上调是患者不良预后的独立危险因素(P<0.05)。[结论]结直肠癌组织中的miR-21表达强度显著上调,并且与肿瘤TNM分期增加、发生淋巴结转移及不良预后有关。

[Objective] To investigate the relationship between miR-21 expression and tumorigenesis in colorectal cancer tissues.[Methods]From January 2015 to September 2016,80 cases of colorectal cancer and 80 cases of paracancerous tissues were selected from our hospital.The miR-21 levels of the two groups were detected by real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).The differences of miR-21 expression in colorectal cancer with different TNM stage,pathological differentiation,pathological type,tumor diameter,tumor location and lymph node metastasis were analyzed.[Results] The expression of miR-21 in colorectal cancer tissues was significantly higher than that in adjacent tissues,and the difference was statistically significant(P<0.05).There were no significant differences in the expression of miR-21 between colorectal cancer tissues with different pathological types,different degrees of differentiation,and different lesion diameters(P>0.05).The expression of miR-21 in colon cancer tissues with TNM stage(stage Ⅲ+Ⅳ),lymph node metastasis,and different prognosis outcomes was significantly higher than that of TNM stage(stage Ⅰ+Ⅱ)and colorectal without lymph node metastasis.The difference in cancer tissues was statistically significant(P<0.05).TNM staging,lymph node metastasis,and up-regulation of miR-21 expression in colorectal cancer patients were independent risk factors for poor prognosis(P<0.05).[Conclusion] The intensity of miR-21 expression in colorectal cancer tissues was significantly up-regulated,and was associated with increased tumor TNM stage,lymph node metastasis,and poor prognosis.