2014-2018年山西省脊髓灰质炎病毒学监测结果分析

Virological surveillance for poliomyelitis in Shanxi,2014–2018

目的评价山西省2014-2018年脊髓灰质炎(脊灰)病毒学监测情况。方法按照世界卫生组织第4版《脊髓灰质炎实验室手册》的要求进行病毒分离和鉴定,对2014-2018年山西省报告的急性弛缓性麻痹(AFP)病例及接触者粪便标本进行脊灰病毒学监测和结果分析,分离到的L20B阳性分离物做型内鉴定,由中国疾病预防控制中心(CDC)病毒病预防控制所国家脊灰实验室对脊灰病毒衣壳蛋白VP1编码区进行核苷酸序列测定和分析。结果2014-2018年山西省CDC脊灰实验室共收到927例AFP病例及其接触者粪便标本共2042份,分离到脊灰病毒21株,分离率为1.03%,11株经鉴定为疫苗相似株,9株为疫苗高变异株,1株为疫苗衍生脊灰病毒;分离到非脊灰肠道病毒103株,分离率为5.04%。结论山西省AFP监测系统敏感,2014-2018年山西省CDC脊灰实验室在AFP病例及接触者粪便标本中未发现脊灰野病毒,山西省继续维持无脊灰状态。

Objective To analyze and evaluate result of virological surveillance for poliomyelitis in Shanxi province from 2014 to 2018.Methods According to the 4 th edition of the polio laboratory manual for virus isolation and identification of World Health Organization,the virological surveillance for poliomyelitis was conducted in the acute flaccid paralysis(AFP)cases and contacts in Shanxi during this period,the detection results of stool samples from the AFP cases and contacts were analyzed.The isolated L20 B positive strains were identified through intra-typic differentiation.The nucleotide sequencing and analyzing of the capsid protein VP1 coding region of poliovirus were performed by National Polio Laboratory,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention(CDC).Results During 2014–2018,Polio Laboratory of Shanxi CDC tested 2042 stool samples of 927 AFP cases and contacts.A total of 21 poliovirus strains were isolated with the isolation rate of 1.03%,in which 11 were Sabin like strains.9 were highly variant vaccine strains and 1 was vaccine-derived poliovirus(VDPV)strain.A total of 103 non-polio enterovirus strains were isolated with the isolation rate of 5.04%.Conclusion The AFP surveillance system of Shanxi is sensitive.During 2014–2018,no wild poliovirus were found from the stool samples of AFP cases and contacts in Shanxi.The polio-free status was kept in Shanxi.