高分子纳米载药体系膀胱灌注治疗在原位膀胱癌动物模型中的初步应用

Application of macromolecule polymer drug loading system’s intravesical therapy in orthotopic bladder cancer model

目的:建立简单高效的小鼠原位膀胱癌动物模型,评估高分子纳米载药体系CPRD在模型中进行膀胱灌注治疗的效果。方法:使用能稳定表达荧光素酶的鼠源膀胱癌细胞MB49-luc,通过癌细胞移植的方法接种到C57BL/6小鼠的膀胱上皮上,通过小动物活体成像技术检测癌细胞的生长状况;将CPRD应用于已经建立的小鼠膀胱癌模型上,分为键合环状RGD多肽的CPRD组、阿霉素(DOX)组和对照组三组进行研究。观察CPRD在小鼠活体身上的抗癌效果。结果:移植MB49-luc的小鼠原位膀胱癌动物模型成瘤率为80%;在小鼠模型膀胱灌注治疗研究中,CRPD组肿瘤抑制效果高于DOX组和对照组(P<0.05),CPRD组膀胱组织内DOX含量也高于DOX组(P<0.05)。结论:成功建立了小鼠原位膀胱癌动物模型,肿瘤生长基本模拟了表浅性膀胱癌的发生、发展过程;高分子纳米载药体系对动物模型中的肿瘤细胞起到了一定的抑制作用。

Objective: To establish a simple and efficient method for an orthotopic mouse bladder cancer model, and investigate the value of macromolecule polymer drug loading system’s intravesical therapy in the model. Method: MB49-luc cell which can produce luciferase was transplanted into C57 BL/6 mouse’s bladder epithelium. Small animals living imaging technology was used to detect the growing of cancer cell in vivo. We used macromolecule polymer drug loading system(CPRD) in orthotopic bladder cancer model which has been established and detected it’s anti-cancer effect. Result: The tumer take rate of orthotopic bladder cancer model was about 80%. In the research of intravesical therapy, the effect of inhibition of tumer growth was higher in CPRD group than that in DOX Group and control group(P<0.05). And the concentration of DOX in bladder was higher in CPRD group than that in DOX group(P<0.05). Conclusion: We succesfully established an orthotopic mouse bladder cancer model, which could approximately simulate the progression of superficial bladder cancer. CPRD’s intravesical therapy could partly inhibit the tumor growth in orthotopic bladder cancer model.