尖吻蝮蛇毒不同蛋白酶水解物的制备及抗氧化活性研究

Preparation and Antioxidant Activity of Different Protease Hydrolysate of Deinagkistrodon acutus Venom

【目的】研究尖吻蝮(Deinagkistrodon acutus)蛇毒的不同蛋白酶水解产物的制备及抗氧化活性。【方法】分别用碱性蛋白酶、中性蛋白酶、木瓜蛋白酶和胰蛋白酶水解尖吻蝮蛇毒,以水解物的水解度、清除自由基活性、金属离子螯合能力和总还原力作为抗氧化活性比较指标来选出最佳水解蛋白酶,并比较该蛋白酶水解物在超滤后得到的不同分子量组分的抗氧化活性大小。【结果】碱性蛋白酶水解物的清除DPPH自由基活性、亚铁离子螯合能力和总还原力均为最佳;该酶解物经超滤得到的3个组分中,分子量为3~10kDa的组分对DPPH自由基和羟基自由基清除能力最强,分子量大于10kDa的组分的亚铁离子螯合能力和总还原力最强。【结论】尖吻蝮蛇毒的不同蛋白酶水解物均具有抗氧化活性,其中用碱性蛋白酶水解物抗氧化活性较强。

[Purposes]The preparation and antioxidant activity of different protease hydrolysate of Deinagkistrodon acutus venom were studied.[Methods]The hydrolysis of D.acutus by four proteases,alkaline protease,neutral protease,papain,and trypsin,the best hydrolyzed protease was selected by comparing the degree of hydrolysis,scavenging free radical activity,metal ion chelation ability and total reducing power,and compare the antioxidant activity of different molecular weight components obtained by ultrafiltration after the protease hydrolysate.[Findings]The alkaline protease hydrolysate is optimal for scavenging DPPH free radicals,ferrous ion chelation ability and total reducing power,among the three components obtained by ultrafiltration,the 3~10 kDa molecular weight components of the three components obtained had the strongest antioxidant activity in DPPH radicals and hydroxyl radicals.The>10 kDa molecular weight fraction has a better effect in the determination of ferrous ion chelation ability and total reducing power.[Conclusions]Different protease hydrolysate of D.acutus have antioxidant activity,and alkaline protease hydrolysate has strong antioxidant activity.