谷氨酰胺对免疫应激肉仔鸡免疫机能的影响

Effect of glutamine on immune function of broilers under immune stress

试验旨在研究日粮中添加谷氨酰胺对免疫应激肉仔鸡模型免疫机能的影响。采用2×2析因试验设计,选用1日龄健康的肉仔鸡1600只,按体重相近的原则随机分为4组,其中第1组为对照组,其他3组为试验组,分别在日粮中添加0.6%的谷氨酰胺(Glutamine,Gln)和进行脂多糖(Lipopolysaccharides,LPS)应激处理,试验期21 d。结果显示,LPS免疫应激提高了肉仔鸡血清中丙二醛(MDA)、皮质醇、TNF-α和IL-8的含量,降低了总超氧化物歧化酶(T-SOD)活性、谷胱甘肽(GSH)活性及免疫球蛋白、IFN-γ、IL-2、IL-6的含量;基础日粮中添加Gln后极显著增强了肉仔鸡血清中GSH活性、极显著或显著提高了IgG、IgA、IgM、IL-2的含量,同时还增强了肉仔鸡血清中T-SOD活性,提高IFN-γ和IL-6的含量,降低了MDA、皮质醇、TNF-α和IL-8的含量,表明日粮中添加谷氨酰胺可以提高肉仔鸡的免疫功能,缓解免疫应激反应。

The experiment was to study the effect of glutamine supplementation on immune function of immune stress broiler model.1600 one-day-old broilers were randomly divided into four groups according to the principle of similar body weight.The first group was the control group and the other three groups were the experimental group.Glutamine were added to the diet and establishment of immune stress model with lipopolysaccharides,the test period were 21 days.The results showed that LPS immune stress increased the serum levels of MDA,cortisol,TNF-α and IL-8,decreased the activities of T-SOD,GSH and the contents of immunoglobulin,IFN-γand IL-2.Gin supplementation in the basic diet significantly enhanced the activity of GSH in serum,the contents of IgG,IgA,IgM and IL-2 significantly increased.It also enhanced the activity of T-SOD in serum,increased the content of IFN-γ and IL-6,and decreased the contents of MDA,cortisol,TNF-α,IL-8.The results showed that the supplementation of glutamine in diet could improve the immune function of broilers and alleviate the immune stress reaction.