摘要
Small molecule-based fluorescence imaging is an indispensable tool for direct visualization and dynamic tracking of important biological species in live samples,due to its excellent sensitivity,remarkable selectivity,easy and fast acquisition,and non-invasive property[1,2].In comparison with one-photon fluorescence imaging,two-photon fluorescence imaging is highly preferable because of pulsed near-infrared(NIR)laser irradiation,resulting in minimal back-ground autofluorescence,high spatial resolution,and deep tissue penetration.
