miRNA451用于法医学血液鉴别的可行性研究

The feasibility study of blood identification by miRNA451

目的探讨m iRNA451是否可以用于法医学血液类物证的鉴别。方法以法医学常见血液类(外周血和月经血)及非血液类(精液、唾液、阴道分泌液)体液样本为研究对象,提取总RNA,以RT-qPCR方法检测1^聊八451的表达,利用5?5522.0进行统计分析。结果111丨1^八451在外周血及月经血中均具有高表达的特点,且表达量显著高于在唾液、精液及阴道分泌液等非血液类物证中的表达量(6组P均<0.05);放置一年的血斑中miRNA451表达量与新鲜血液无显著差异。结论miRNA451在血液类体液样本中的表达水平显著高于非血液类体液样本,且稳定性良好,可作为法医学血液类体液样本鉴别的良好分子标记物。

Objective To work on the practicability of performing blood identification by detecting miRNA451using RT-qPCR. Methods Total RNA was extracted from common forensic blood samples (peripheral blood and menstrualblood) and non-blood (semen, saliva, vaginal secretion) body fluids samples. The expression of miRNA451 was detectedby RT-qPCR. SPSS 22.0 was used for statistical analysis. Results The expression values of miRNA451 in peripheral bloodsamples and menstrual blood samples were higher than that in saliva, semen and vaginal secretion (all of the P values <0.05) samples;the expression of miRNA451 in peripheral blood samples and menstrual blood samples had no statisticalsignificance (P > 0.05);the expression of miRNA451 in old blood stain samples and fresh peripheral blood samples had nostatistical significance (P > 0.05). Conclusion miRNA451 can be used as the human blood specific molecular and is stableenough to identify human blood samples.