雷公藤甲素对雄激素非依赖性前列腺癌细胞株的影响

The effect of triptolide on androgen independent prostate cancer cell line

目的 探讨雷公藤甲素(TPL)对雄激素非依赖前列腺癌细胞株LNCaP-AI的影响,并初步探讨其机制.方法 用(0、12.5、25、50和100nmol/L)浓度的TPL处理本实验组诱导的雄激素非依赖细胞株LNCaP-AI后,采用CCK-8 检测LNCaP-AI细胞株的存活率、逆转录荧光PCR(RT-PCR)分析雄激素受体(AR)的表达情况,按所筛选出的TPL浓度进行后续实验.实验分为2组:control组:雄激素非依赖细胞株LNCaP-AI;TPL组:雄激素非依赖细胞株LNCaP-AI模型组中加入100nmol/L浓度 TPL.24h后,RT-PCR分析AR以及AR靶基因TNFAIP8的表达情况.结果 不同浓度 TPL 处理后,LNCaP-AI细胞的存活率下降,且下降与TPL呈现浓度依赖性.AR的mRNA相对表达量也出现剂量依赖性的下调.100nmol/L浓度的TPL对细胞的抑制作用最强.TPL组相对于control组,TPL给药作用24h后可降低细胞AR mRNA的相对表达量(P<0.05),以及AR靶基因TNFAIP8 mRNA的相对表达量(P<0.05).结论 TPL抑制雄激素非依赖细胞株LNCaP-AI增殖可能与其降低AR以及AR靶基因TNFAIP8的表达有关.

Objective To investigate the effect of triptolide TPL on androgen independent prostate cancer cell line LNCaP-AI and discuss its mechanism preliminarily.Methods The androgen independent cell line LNCaP-AI was treated with TPL(0,12.5,25,50 and 100nmol/L).The survival rate of LNCaP-AI cell line was detected by using CCK-8 and expression level of androgen receptors(AR)was analyzed by PCR(RT-PCR).Following experiments were carried out according to selected TPL concentration.The experiment was divided into two groups:Control Group:androgen-dependent cell line LNCaP;TPL Group:androgen-independent cell line LNCaP-AI model group with 100nmol/L TPL.The expression levels of AR and its target gene TNFAIP8 were detected by RT-PCR after 24 hours.Results The survival rate of LNCaP-AI cells as well as mRNA level was decreased significantly after various concentrations TPL treatment,and the decrement was dose-dependent with TPL.TPL with 100 nmol/L concentration had the strongest inhibitory effect on cells.Compared to Control Group,mRNA content of AR and its target gene was significantly decreased(P<0.05)in TPL Group after 24-hours TPL delivery.Conclusions The inhibition of TPL on the proliferation of androgen independent cell line LNCaP-AI is possibly related to the inhibition of AR and its target gene TNFAIP8 expression.