基于四氧化三铁修饰传感器检测茶叶中儿茶酚

Detection of Catechol in Tea Based on Tri-iron Oxide Modified Sensor

制备了Fe3O4磁性纳米材料,以玻碳电极(Glassy carbon electrode, GCE)为工作电极制备了Fe3O4/GCE传感器,并用于茶叶中儿茶酚的定量分析。结果表明:Fe3O4具有较好的导电性,能够有效地促进电子的转移,增加响应电流,提高电极灵敏度。利用Fe3O4/GCE对儿茶酚进行定量分析,儿茶酚浓度与其氧化峰电流在5~120μmol/L范围内呈现良好的线性关系,线性方程为Y=0.015 2X+0.546 4, R2=0.998 4,相关性较好,检出限为0.35μmol/L,符合试验要求,且该传感器重复性较好。利用绘制好的标准曲线对茶叶中的儿茶酚进行定量分析,其结果显示茶叶中儿茶酚含量为10.62 mg/g,与HPLC检测结果相一致,可信度较高,可用于茶叶中儿茶酚的快速检测分析。

Fe3O4 magnetic nanomaterials were synthesized. The Fe3O4/GCE sensor was prepared by using glassy carbon electrode(GCE) as working electrode and used for quantitative analysis of catechol in tea. The results showed that Fe3O4 had good conductivity, could effectively promote the transfer of electrons, increases the response current and improves the sensitivity of the electrode. Catechol in tea was detected by Fe3O4/GCE sensor. The study found that there was a wide linear from 5~120 μmol/L between the oxidation peak current and the concentration of catechol, the equation was Y=0.015 2 X+0.546 4, R2=0.998 4, the detection limit for catechol was 0.35 μmol/L, which could afford for the requirement of the application, and the sensor had a good repeatability. Quantitative analysis of catechol in tea was carried out by using the standard curve, the results showed that the content of catechol in tea was 10.62 mg/g, which was consistent with the results of HPLC and had high reliability. It could be used for rapid detection and analysis of catechol in tea.