重症肺炎与炎性因子及 TBX21基因位点rs16947078相关性分析

Analysis of the correlation between severe pneumonia and inflammatory factors and TBX21 locus rs16947078

目的 分析重症肺炎与炎性因子和TBX21基因位点rs16947078的相关性.方法 选取温岭市第四人民医院2017年3月至2019年3月收治的重症肺炎患者71例;另选择该院同期收治的普通肺炎患者83例.采用酶联免疫吸附法(ELISA 法)检测白细胞介素6(IL-6)、白细胞介素8(IL-8)、白细胞介素18(IL-18)和肿瘤坏死因子α(TNF-α)水平变化;采用实时荧光定量PCR法检测TBX21基因位点rs16947078基因型和基因分布.结果 重症肺炎组肺部感染评分(CPIS)(6.57 ± 1.20)分,高于普通肺炎组的(4.93 ± 1.05)分(t=9.045,P<0.05);重症肺炎组的 IL-6(65.42 ± 13.25)ng/L、IL-8(457.87 ± 45.36)ng/L、IL-18(87.59 ± 15.46)ng/L和TNF-α(58.98 ± 13.25)ng/L,均高于普通肺炎组的(36.71 ± 8.98)ng/L、(218.98 ± 32.45)ng/L、(54.37 ± 12.10)ng/L和(37.54 ± 7.90)ng/L(t=15.926、37.959、14.946、12.394,均P<0.05);重症肺炎组TBX21基因位点rs16947078等位基因G分布(40.85%)高于普通肺炎组(18.07%)(χ^2 =9.724,P<0.05);重症肺炎组TBX21基因位点rs16947078基因频率GG(36.62%)高于普通肺炎组(12.05%),而基因频率AA(52.11%)低于普通肺炎组(72.29%)(χ^2 =12.898、6.682,均P<0.05).结论 重症肺炎患者IL-6、IL-8、IL-18和TNF-α水平明显升高,且认为TBX21基因位点rs16947078等位基因A可能为重症肺炎易感基因.

Objective To analyze the correlation between severe pneumonia and inflammatory factors and TBX21 locus rs16947078.Methods From March 2017 to March 2019,in the Fourth People's Hospital of Wenling,71 patients with severe pneumonia were selected,and 83 patients with general pneumonia were selected during the same period.The levels of interleukin-6(IL-6),interleukin-8(IL-8),interleukin-18(IL-18)and tumor necrosis factor-alpha(TNF-α)were detected by enzyme-linked immunosorbent assay(ELISA).The genotype and gene distribution of rs16947078 at TBX21 locus were detected by real -time fluorescence quantitative PCR.Results The score of CPIS in the severe pneumonia group was higher than that in the common pneumonia group [(6.57 ± 1.20)points and(4.93 ± 1.05)points](t=9.045,P<0.05).The serum levels of IL-6 [(65.42 ± 13.25)ng/L],IL -8 [(457.87 ± 45.36)ng/L],IL -18 [(87.59 ± 15.46)ng/L] and TNF -α [(58.98 ± 13.25)ng/L] in the severe pneumonia group were higher than those in the general pneumonia group [(36.71 ± 8.98)ng/L,(218.98 ± 32.45)ng/L,(54.37 ± 12.10)ng/L and(37.54 ± 7.90)ng/L](t =15.926,37.959,14.946,12.394,all P<0.05).The G distribution of rs16947078 allele at TBX21 locus in the severe pneumonia group(40.85%)was higher than that in the normal pneumonia group(18.07%)(χ^2 =9.724,P<0.05).The GG(36.62%)of TBX21 locus rs16947078 in the severe pneumonia group was higher than that in the common pneumo-nia group(12.05%),while the AA(52.11%)of TBX21 locus in the severe pneumonia group was lower than that in the common pneumonia group(72.29%)(χ^2 =12.898,6.682,P<0.05).Conclusion The levels of inflammatory factors IL-6,IL -8,IL -18 and TNF -α in patients with severe pneumonia aresignificantly increased,and the TBX21 locus rs16947078 allele A may be a susceptible gene for severe pneumonia.