溪黄草药材质量控制含量测定方法研究

Study on quality control content determination method of Herba Rabdosiae Serrae

目的:建立同时测定溪黄草药材中齐墩果酸、熊果酸含量的方法。方法:色谱柱为CAPCELL PAK C18MGⅡ(以十八烷基硅烷键和硅胶为填充柱),流动相为乙腈-甲醇-0.6%乙酸铵(67∶12∶21),流速为1 mL/min,检测波长为210 nm,柱温为30℃,进样量为20μL。结果:齐墩果酸和熊果酸分别在5.066~50.664μg/mL,9.735~97.352μg/mL范围内呈现良好的线性关系(r=0.998);精密度、稳定性、重复性试验的RSD<2.0%;平均回收率分别为99.78%(RSD=0.56%)和100.68%(RSD=0.67%)。结论:该方法操作简单、重复性好、准确度高,可以用于溪黄草药材的质量控制。

Objective:To establish the method for simultaneous determination of oleanolic acid and ursolic acid in traditional Chinese medicine of Herba Rabdosiae Serrae.Methods:The chromatographic column was CAPCELL PAK C18 MGⅡ(with octadecylsilane chemically bonded silica as the packed column),and it was adopted with the mobile phase of acetonitrile-methanol-0.6%ammonium acetate(67:12:21).The detection wavelength was set at 210 nm with the flow rate of 1 mL·min^-1,the column temperature of 30℃and the injection volume of 20μL.Results:Oleanolic acid and ursolic acid exhibited satisfied linear relationship in the range of 5.066~50.664μg•mL^-1 and 9.735~97.352μg·mL^-1,respectively(r=0.998).Meanwhile,the RSD of precision,stability and repeatability were less than 2.0%.The average recovery rates were 99.78%(RSD=0.56%)and 100.68%(RSD=0.67%),respectively.Conclusion:The proposed method of HPLC was simple,reproducible and accurate,and can be applied for quality control of medicinal material of Herba Rabdosiae Serrae.