酶辅助提取纯化芹菜总黄酮的工艺研究

Study on enzyme-assisted extraction and purification of total flavonoids from Apium graveolens L.

目的:筛选纤维素酶辅助提取芹菜总黄酮的最优提取工艺。方法:采用单因素试验方法分别考察乙醇浓度、料液比、提取时间、提取次数以及酶用量、pH值、酶解时间、酶解温度对芹菜总黄酮提取率的影响,同时对以上因素分别进行L9(3^4)正交试验,得到酶辅助提取芹菜总黄酮的最佳提取工艺。结果:最佳工艺条件是纤维素酶用量7.0%、pH值4.4、酶解温度45℃、酶解时间1 h、乙醇浓度60%、料液比1∶20、提取时间2 h、提取次数1次。在此工艺下,芹菜总黄酮的提取率为4.17%。结论:加入纤维素酶可以有效提高芹菜总黄酮的提取率。

Objective:To screen the optimal extraction process of total flavonoids from Apium graveolens L.by cellulase-assisted method.Methods:By single factor test,the effects of ethanol concentration,solid-liquid ratio,extraction time,number of extractions and enzyme dosage,pH,enzymatic hydrolysis time and enzymatic hydrolysis temperature were observed.Based on the L9(3^4)orthogonal test,the best extraction process of enzyme-assisted extraction of total flavonoids from Apium graveolens L.Results:The optimum conditions were:cellulase dosage 7.0%,pH 4.4,enzymatic hydrolysis temperature 45°C,enzymatic hydrolysis time 1 h,ethanol concentration 60%,solid-liquid ratio 1:20,extraction time 2 h,extraction times 1 time.Under this process,and the extraction rate of total flavonoids from Apium graveolens L.was 4.17%.Conclusion:The extraction rate of total flavonoids from Apium graveolens L.can be effectively increased by adding to the cellulose.