摘要
High-quality and disease-resistant male sterile lines have great potential for applications in hybrid rice breeding.We introduced specific mutations into the TMS5,Pi21,and Xa13 genes in Pinzhan intermediate breeding material using the CRISPR/Cas9 multiplex genome editing system.We found that the transgenefree homozygous triple tms5/pi21/xa13 mutants obtained in the T1 generation displayed characteristics of thermosensitive genic male sterility(TGMS)with enhancedresistance to rice blast and bacterial blight.Our study provides a convenient and effective way of converting breeding intermediate material into TGMS lines through multiplex gene editing,which could significantly accelerate the breeding of sterile lines.
High-quality and disease-resistant male sterile lines have great potential for applications in hybrid rice breeding. We introduced specific mutations into the TMS5, Pi21, and Xa13 genes in Pinzhan intermediate breeding material using the CRISPR/Cas9 multiplex genome editing system. We found that the transgenefree homozygous triple tms5/pi21/xa13 mutants obtained in the T1 generation displayed characteristics of thermosensitive genic male sterility(TGMS) with enhancedresistance to rice blast and bacterial blight. Our study provides a convenient and effective way of converting breeding intermediate material into TGMS lines through multiplex gene editing, which could significantly accelerate the breeding of sterile lines.
基金
supported by the National Natural Science Foundation of China(31601285)
the Central Public-interest Scientific Institution Basal Research Fund of China National Rice Research Institute(2017RG002-4)
