青春期前高脂饮食诱导的多囊卵巢综合征大鼠氧化应激和低度炎症变化

The changes of oxidative stress and low-grade inflammation of pre-pubertal rats with polycystic ovary syndrome induced by high-fat diet

目的:探讨青春期前高脂饮食诱导的多囊卵巢综合征(PCOS)大鼠模型中氧化应激(OS)和低度炎症变化。方法:将青春期前雌性大鼠随机分组,对照组接受正常饮食(n=18),诱导组接受高脂饮食(n=18),连续喂养105d,构建PCOS动物模型。检测两组大鼠喂养第49d、77d、105d时卵巢组织双氧化物歧化酶(SOD)、丙二醛(MDA)和脂质过氧化物(LPO)和血清高敏C-反应蛋白(hsCRP)水平。结果:诱导组动情周期呈非规律性变化,卵巢重量显著增加,卵巢组织出现多个囊状扩张卵泡、小卵泡及闭锁卵泡,囊状卵泡数、卵泡直径、卵泡膜厚度增加,黄体数量下降,糖耐量、空腹胰岛素水平、HOMA-IR、QUICK、总胆固醇、雌二醇(E2)、孕酮(P)、睾酮(T)水平显著变化;对照组SOD高于诱导组, MDA、LPO及hsCRP低于诱导组(均P<0.05)。诱导组大鼠SOD与MDA、LPO、hrCRP、HOMA-IR、空腹胰岛素呈负相关,MDA与LPO、hrCRP、HOMA-IR呈正相关,LPO与hrCRP和T呈正相关,hrCRP与T呈正相关(均P<0.05)。结论:青春期前高脂饮食诱导PCOS大鼠中OS和低度炎症(hsCRP)发展与疾病发生较一致,提示OS和低度炎症可能与PCOS有关。

Objective:To investigate the changes of oxidative stress(OS)and low-grade inflammatory of pre-pubertal rats with polycystic ovary syndrome(PCOS)induced by high-fat diet.Methods:36 pre-pubertal female rats were randomly divided into group A and group B(18 case in each group).The rats in group A were given normal diet fed for 105 days,and the rats in group B were given high-fat diet fed for 105 days to construct PCOS animals’model.The levels of superoxide dismutase(SOD),malondialdehyde(MDA),lipid peroxidation(LPO)in ovarian tissue and serum high-sensitivity C-reactive protein(hsCRP)of all rats were detected on 49 th d,77 th d and 105 th d,respectively.Results:In group B,the estrous cycle of rats had changed irregularly,their ovary weight were also increased significantly,and there were multiple cystic dilated follicles,small follicles and atresia follicles in ovarian tissue of them,and the number of cystic follicles,follicular diameter,theca layer thickness and corpus luteum had decreased significantly,and the levels of glucose tolerance,fasting insulin,HOMA-IR,QUICK,total cholesterol,estradiol(E2),progesterone(P)and testosterone(T)had also changed significantly(P<0.05).The SOD level of rats in group A was significant higher than that of rats in group B,but the levels of MDA,LPO and hsCRP of rats in group A were significant lower(P<0.05).In group B,the SOD level of rats was negatively correlated with their levels of MDA,LPO,hrCRP,HOMA-IR and fasting insulin,MDA level of rats was positively correlated with their levels of LPO,hrCRP and HOMA-IR(P<0.05),the LPO level of rats was positively correlated with their levels of hrCRP and T(P<0.05),and the hrCRP level of rats was positively correlated with their T level(P<0.05).Conclusion:The low-grade inflammation occurance in pre-pubertal rats with PCOS induced by high-fat diet is consistent with the disease development of PCOS,so it is suggested that OS and low-grade inflammation may also be associated with rat model with PCOS.