miRNA-630在鼻咽癌中的表达情况及与不良预后的相关性分析

Expression and prognosis analysis of miRNA-630 in nasopharyngeal carcinoma patients

目的探讨微小RNA(miRNA)-630在鼻咽癌中的表达情况及与鼻咽癌患者预后的相关性。方法从NCBI-GEO网站中下载了GSE70970、GSE43329和GSE12452共3个独立的鼻咽癌芯片数据,使用Graphpad prism 7对miRNA-630的表达差异进行统计分析;对miRNA-630的表达进行了受试者工作特征曲线(ROC)和Kaplan-Meier生存曲线分析。采用SPSS22.0对miRNA-630表达量、肿瘤病理(T)和淋巴结转移(N)指标建立了单因素和多因素COX风险比例模型。采用RT-qPCR检测鼻咽癌组织和正常组织标本中miRNA-630的表达情况。培养CEN2细胞,分为3组,分别仅加入磷酸盐缓冲液(对照组)、转染空载(空载组)和miRNA-630(miRNA-630组),采用MTT法测定细胞生长情况。最后,预测并验证miRNA-630在鼻咽癌中致癌的机制。结果miRNA-630在鼻咽癌组织中的表达量明显高于正常组织(P<0.05)。转染miRNA-630载体48、72、96h后,miRNA-630组细胞水平明显高于对照组和空载组(P<0.05)。miRNA-630组的CNE2细胞中TP53INP2基因和蛋白表达水平明显低于对照组和空载组(P<0.05)。结论miRNA-630在鼻咽癌中高表达,与不良预后相关,miRNA-630的高表达可促进鼻咽癌细胞的增殖,可作为鼻咽癌预后的独立预测因子;其作用机制可能与靶向抑制TP53INP2有关。

Objective To investigate the expression of microRNA(miRNA)-630 and its correlation with prognosis in nasopharyngeal carcinoma(NPC).Methods GSE70970,GSE43329 and GSE12452 three independent nasopharyngeal carcinoma chip data were downloaded from the NCBI-GEO website,and the expression difference of miRNA-630 was statistically analyzed using Graphpad prism 7.The difference between the groups was analyzed by t test,the expression of miRNA-630 was analyzed by receiver operating characteristic curve(ROC)and Kaplan-Meier survival curve.A single-factor and multi-factor COX risk scale model was established using SPSS22.0 for miRNA-630 expression,tumor pathology(T)and lymph node metastasis(N)indicators.In addition,nasopharyngeal carcinoma tissues and normal tissue samples were collected,and the expression of miRNA-630 was detected by RT-qPCR.CEN2 cells were cultured,and were divided into three groups.Cells in three groups were added phosphate buffer(control group),blank load transfection(blank load group)and miRNA-630(miRNA-630 group),respectively.MTT was used to test the cell growth.Finally,the mechanism of miRNA-630 up-regulation in NPC was predicted.Results The expression of miRNA-630 in NPC was significantly higher than normal tissues(P<0.05).The expression of miRNA-630 was significantly higher than that of control group 48,72 and 96 h after transfection.And the mRNA and protein expression of TP53INP2 in CNE2 cells of miRNA-630 group was significantly lower than that of control group and blank load group.Conclusion Overexpression of miRNA-630 in NPC is associated with poor prognosis and could be served as an independent prognostic factor.The possible mechanism may be related with inhibition with TP53INP2.