便携式食品直链和支链淀粉速测仪的研制

Development of Portable Speed Measuring Apparatus for Amylose and Amylopectin in Food

依据等吸收双波长消去比色法原理,研制了便携式食品直链和支链淀粉速测仪,并进行两种淀粉含量的检测.验证试验结果表明线性关系(R 2>0.999)、精密度(RSD%<0.7%)、稳定性(RSD%<1.5%)、重复性(RSD%<2.0%)、重现性(实验室内RSD%<2.5%,实验室间RSD%<4.2%)、回收率(平均回收率高于90%,RSD%<2.5%)和系统适用性(设备RSD%<2.0%,设备之间RSD%<3.5%)良好.其检测过程简单、快速、准确,两种淀粉的检测和参比波长集成在仪器装置单片机系统中,试验时可直接使用,无需再次对其进行设定.两种淀粉的吸光度差值ΔA直和ΔA支只需测定两次,节省了操作时间,提高了样品的检测效率.便携式速测仪的研制为快速、准确地检测大批量样品中两种淀粉提供了有力的技术保障.

According to the principle of equal absorption dual-wavelength elimination colorimetric method,two starch contents were determined by the developed portable speed measuring apparatus for amylose and amylopectin in food.The validation test results indicated that the results of linear response ranges(R 2>0.999),precision(RSD%<0.7%),stability(RSD%<1.5%),repeatability(RSD%<2.0%),reproducibility(RSD%<2.5%in the same laboratory and RSD%<4.2%in different laboratories),recovery(average recovery was more than 90%,RSD%<2.5%),system applicability(RSD%<2.0%for each apparatus and RSD%<3.5%between different apparatuses)were good,respectively.The detection process is simple,fast and accurate,the detection wavelength and reference wavelength of the two starches which were integrated in the single-chip microcomputer system of the instrument device were used directly.So these parameters were no need to be set again during the experiment and only two times of the absorbance differences were need to be determined.The operation time was saved and the sample detection efficiency was improved.The development of this portable speed measuring apparatus provides a powerful technical guarantee for the rapid and accurate detection of two different starches as in large batches of samples and it is an important technological innovation in the functional development of the function of speed measuring apparatus for starch.