硅罗丹明荧光探针的研究进展

Progress on Si-Rhodamine Fluorescent Probes

近年来,罗丹明染料因其良好的荧光性能,在化学和生物化学领域倍受关注。但罗丹明染料的荧光波长通常都小于600 nm,具有较强的生物背景荧光的干扰,很大程度上限制了其在生物成像等领域的实际应用。将罗丹明母核的氧原子替换为硅原子,所发展的硅罗丹明不仅具备传统罗丹明荧光染料的优点,还可将荧光波长红移至近红外波段,很好地解决了罗丹明荧光波长较短的问题。本文主要综述了硅罗丹明荧光探针的研究进展,重点聚焦该荧光团波长红移原理、荧光波长和荧光量子产率的调控,以及硅罗丹明探针的设计机理与应用。

Rhodamine dyes are widely used as fluorescent probes owing to their high absorption coefficient, broad fluorescence in the visible region of electromagnetic spectrum, high fluorescence quantum yield and photostability. A great interest in the development of new synthetic procedures for preparation of rhodamine derivatives has arisen in recent years because the probe must be covalently linked to another(bio)molecule or surface for most applications. To date, the emission wavelength of rhodamine dyes is always less than 600 nm, which restricts the practical application of rhodamines in living systems due to the serious background fluorescence interference. In this critical review the strategies for modification of rhodamine dyes and a discussion on the variety of applications of these new derivatives as fluorescent probes are given. Upon replacing the oxygen bridge atom by silicon, the resulting Si-rhodamines have been developed as novel near-infrared(NIR) fluorescent cores with remaining the key advantages of conventional rhodamine chromophore, and also generating the bathochromic shift in their emission wavelengths to NIR region. Based on the essential properties of Si-rhodamine, this paper mainly focuses on the principle of the red shift in the emission wavelength, the regulation of fluorescence wavelength and fluorescence quantum yield, and the design mechanism and application of Si-rhodamine fluorescent probes.