阿胶对环磷酰胺所致免疫抑制小鼠的免疫保护作用

Immunoprotective of Colla Corii Asini on immune function in immunosuppressed mice induced by cyclophosphamide

目的探究不同品牌阿胶对环磷酰胺(Cy)所致免疫功能低下小鼠的免疫调节作用。方法ICR小鼠随机分成5组:对照组、Cy组、A品牌阿胶组、B品牌阿胶组和C品牌阿胶组。Cy腹腔注射致免疫抑制,3组阿胶组小鼠均灌服5%不同品牌阿胶溶液,7d后,测定并比较各组小鼠的脾脏和胸腺指数、小鼠血清溶血素的含量,ELISA检测小鼠血清肿瘤坏死因子α(TNF-α)和粒细胞-巨噬细胞集落刺激因子(GM-CSF)的表达,RT-PCR检测各组小鼠脾脏TNF-α和GM-CSF mRNA的表达。结果与Cy组相比,A、B、C 3种品牌阿胶小鼠的胸腺和脾脏指数均显著升高,小鼠血清抗体的含量显著提高,其中A、B品牌阿胶小鼠提高更为显著(P<0.001);与Cy组相比,A、B、C 3种品牌的阿胶小鼠血清中TNF-α和GM-CSF的表达显著增加,小鼠脾脏TNF-α和GM-CSF mRNA的表达升高,其中A品牌阿胶小鼠提高更为明显(P<0.001)。结论3种品牌阿胶对Cy导致的免疫功能低下小鼠均有免疫保护作用,提高尤以A品牌阿胶效果最好。

Objective To explore the immunoregulatory function of different brands of Colla Corii Asini in immunosuppressed mice induced by cyclophosphamide.Methods ICR mice were randomly divided into five groups:control group,cyclophosphamide(Cy)group,and brand A,B and C Colla Corii Asini group.The immunosuppression was induced by receiving intraperitoneal injection of Cy.The mice in Colla Corii Asini group received intragastric administration of 5%brand A,B and C Colla Corii Asini solution every day.After 7 days,the thymus and spleen index,the content of serum hemolysin were analyzed.The expression of tumor necrosis factorα(TNF-α)and the granulocyte-macrophage colony-stimulating factors(GM-CSF)in peripheral blood was detected by ELISA.RT-PCR was used to detect the changes of TNF-αand GM-CSF mRNA expression in spleen cells of mouse.Results Compared with the Cy group,three brands of Colla Corii Asini increased the thoracic(spleen)index,the serum hemolysin level,while Brand A and B increased them more significantly(P<0.001).Compared with the Cy group,three brands of Colla Corii Asini increased the expression of TNF-αand GM-CSF in peripheral blood and spleen cells of mouse,while Brand A increased them more significantly(P<0.001).Conclusion Three brands of Colla Corii Asini can improve the immune function in immunodepressed mice induced by Cy.Among them,brand A has the best effect.