新诊断HIV-1抗体阳性者的WB带型及首次CD4^+T细胞检测分析

WB band type and the first CD4^+T lymphocyte detection results of newly diagnosed HIV-1 antibody positive people

目的分析2016-2018年台山市HIV-1抗体阳性者WB带型及首次CD4^+T细胞检测结果,探讨新诊断HIV-1抗体阳性者的WB带型分布变化规律。方法采用WB蛋白印迹法检测HIV-1抗体带型,流式细胞仪检测CD4^+T细胞,对新诊断患者的WB带型特征及首次CD4^+T细胞检测结果分析。结果 311例HIV-1抗体阳性者中,不同性别比较WB带型出现率差异无统计学意义,≥60岁年龄组gag基因编码条带p17的出现率低于其它两个年龄组(P<0.05),性行为感染者gag基因编码条带p55的出现率低于静脉吸毒感染者(P<0.05), WB条带gp160、gp120、gp41、p66、p51、p31、p24维持较高出现率,全带型、缺失p55、缺失p55+p17的带型是3个不同免疫状况主要带型,在艾滋病期全带型出现率(15.5%)明显降低,缺失p55+p17组在艾滋病期缺失率(31.0%)明显高于其它两组(P<0.05)。结论 HIV-1抗体阳性者WB带型分布在不同年龄、不同传播途径和不同免疫状况的人群间差异有统计学意义,gag带型p55和p17同时转阴与艾滋病发病有关,有助于了解患者的疾病进展。

Objective To explore the change rule of WB band type by analyzing the results of WB band type and the first CD4^+T lymphocytes in Taishan City, 2016-2018. Methods Band patterns of HIV-1 antibodies were analyzed by western bolt, CD4^+T cells were counted by flow cytometer. The characteristics of WB band type in newly diagnosed patients and the first CD4^+T cell test results were analyzed. Results Among the 311 HIV-1 antibody positive people, the occurrence of WB band had no statistical difference among different gender;the occurrence of p17 band(≥60 years old), encoding by gag gene,was significantly lower than the other two age groups(P<0.05);the occurrence of p55 band, encoding by gag gene, was significantly lower in sexual behavior compared with drug addicts(P<0.05). The WB bands, including gp160, gp120, gp41,p66, p51, p31, p24 showed high occurrence. Full-band type, missing p55 band type and missing p55+p17 band type were the common band patterns of WB among the three different immune status. The occurrence of full-band in AIDS group(15.5%)was significantly lower, while the occurrence of missing p17+p55 band(31.0%)was higher than the other two groups(P<0.05).Conclusions The distribution of WB band was significantly different among people who are different ages, different route of transmission or from different immune status, gag band p55 and p17 turning to be negative are correlated with the incidence of AIDS, which is helpful to understand the progress of disease among the patients.