miRNA-466检测在肺癌诊断中的临床价值研究

Clinical value of miRNA-466 in the diagnosis of lung cancer

目的检测海南地区肺癌患者与非肺癌患者血液样本中miRNA-466的表达水平,分析其在肺癌诊断中的临床价值。方法选取肺癌患者50例设为病例组,非肺癌患者50例设为对照组,提取研究对象血液中的miRNA,采用real-time PCR技术检测miRNA-466表达水平。分析病例组和对照组之间miRNA-466表达水平的差异,病例组中不同年龄、性别、饮酒和吸烟状况对miRNA-466表达水平的影响。并利用二元Logistic回归方法,探究各个因素对肺癌发病的影响,确定肺癌发病的高危因素。构建受试者工作特征(ROC)曲线评估miRNA-466对肺癌诊断的价值。结果肺癌组患者血液中miRNA-466表达水平高于对照组,差异有统计学意义(P<0.05)。在肺癌组中,有吸烟史患者miRNA-466的表达水平高于无吸烟史患者,差异有统计学意义(P<0.001)。Logistic回归结果显示,miRNA-466的高表达水平是肺癌发生的一个高危因素(P<0.05)。ROC曲线下面积(AUC)为0.615,灵敏度为46%,特异度为80%,约登指数为0.260,对应的临界值为0.265。结论 miRNA-466有望作为诊断肺癌的生物标志物。

Objective To detect the expression level of miRNA-466 in blood samples from patients with lung cancer and other respiratory diseases in Hainan, and further analyze its clinical value in the diagnosis and treatment of lung cancer.Methods Totally 50 patients with lung cancer were selected as the case group, and 50 patients with non-lung cancer were selected as the control group. The miRNA in the blood of the study subjects was extracted, and the expression level of miRNA-466 was detected by real-time PCR.The differences in miRNA-466 expression levels between cases and controls, and the effects of different age, sex, alcohol consumption, and smoking status on the expression level of miRNA-466 in the case group were analyzed.The binary logistic regression method was used to explore influence factors on the incidence of lung cancer, and to determine risk factors of lung cancer.To construct a receiver operating characteristic(ROC) curve to assess the value of miRNA-466 in the diagnosis of lung cancer.Results The expression level of miRNA-466 in the blood of patients with lung cancer was significantly higher than that of the control group(P<0.05).In the lung cancer group, the expression level of miRNA-466 in patients with smoking history was significantly higher than that in patients without smoking history, and the difference was statistically significant(P<0.001).Logistic regression results showed that the expression level of miRNA-466 was a high risk factor for lung cancer(P<0.05).The area under the ROC curve(AUC) was 0.615, the sensitivity was 46%, the specificity was 80%, the Yoden index was 0.260, and the corresponding cutoff value was 0.265.Conclusions miRNA-466 may be a biomarker for the diagnosis of lung cancer.