摘要
采用形态学观察和ITS序列分析相结合的方法,将采自云南丽江的野生子实体鉴定为鳞皮新香菇(Neolentinus lepideus)。通过组织分离和菌种纯化,获得菌株JZ505,对该菌株的菌丝培养条件进行了探究并进行了人工栽培实验。研究结果表明该菌株的最适碳源为果糖或蔗糖,最适氮源为酵母粉,适宜生长温度为25℃,最适pH 5.0~6.0,经栽培成功得获得了该菌株的子实体。
A wild Neolentinus lepideus strain JZ505 was isolated from a wild mushroom fruiting body collected in Lijiang,Yunnan Province based on its exterior fruiting body morphology.Using ITS sequence analysis,JZ505 was confirmed to be N.lepideus.The cultivation results showed that the optimum carbon source was fructose or sucrose;the optimum nitrogen source was yeast extract;the optimum growth temperature was 25℃;and the optimum pH value range was 5.0-6.0.JZ505 developed fruiting bodies under the above mentioned optimal conditions.
作者
杨珍福
余金凤
马明
岳万松
冯云利
刘绍雄
罗孝坤
郭相
YANG Zhenfu;YU Jinfeng;MA Ming;YUE Wansong;FENG Yunli;LIU Shaoxiong;LUO Xiaokun;GUO Xiang(Kunming Edible Fungi Institute of All China Federation of Supply and Marketing Cooporatives,Kunming,Yunnan 650221,China)
出处
《食用菌学报》
CSCD
北大核心
2019年第4期72-77,共6页
Acta Edulis Fungi
基金
云南省科技厅创新引导与科技型企业培育计划(2018DC030)
云南省科技厅基础研究计划(2018FD170)
关键词
鳞皮新香菇
分离
鉴定
培养特性
Neolentinus lepideus
isolation
identification
cultivation characteristics
