青蒿素抑制人肝癌细胞Huh7和SMMC-7721增殖及其机制

Inhibitory effect and its mechanism of artemisinin on the proliferation of hepatoma Huh7 and SMMC-7721 cells

目的探讨青蒿素抑制肝癌细胞Huh7和SMMC-7721增殖的作用及其机制。方法不同浓度的青蒿素与人肝癌细胞Huh7和SMMC-7721共培养24、48、72 h后,采用Cell viability法检测细胞的增殖活性;细胞克隆实验检测细胞集落的抑制情况;流式细胞仪检测细胞的凋亡情况;Western Blot法检测细胞内蛋白水平的变化。结果Cell viability与细胞克隆实验的结果表明:与空白对照比较,青蒿素能够增加肝癌细胞Huh7和SMMC-7721的凋亡率。青蒿素通过抑制mTOR信号通路,进而抑制肝癌细胞Huh7和SMMC-7721的增殖。结论青蒿素能够抑制肝癌细胞Huh7和SMMC-7721的增殖,诱导其凋亡,且通过抑制mTOR信号通路活化抑制肝癌细胞Huh7和SMMC-7721的增殖,进而发挥抗肿瘤作用。

OBJECTIVE To study the inhibitory effect and its mechanism of artemisinin on the proliferation of Huh7 and SMMC-7721 cells.METHODS Different concentrations of artemisinin were co-cultured with human hepatoma Huh7 and SMMC-7721 cells for 24 h,48 h and 72 h.Cell proliferation activity was measured using Cell viability assay.The inhibition,and apoptosis of cells was detected by Cell cloning assay and flow cytometry,respectively.The changes in intracellular protein levels were detected by Western Blot assay.RESULTS The results of Cell viability assay and Cell cloning assay showed that artemisinin could increase the apoptosis rate of Huh7 and SMMC-7721 cells compared with the control group.Artemisinin inhibits the proliferation of HCC cells Huh7 and SMMC-7721 by inhibiting the mTOR signaling pathway.CONCLUSION Artemisinin can inhibit the proliferation and induce apoptosis of Huh7 and SMMC-7721,and inhibit the proliferation of Huh7 and SMMC-7721 by inhibiting the activation of mTOR signaling pathway,thus exerting the anti-tumor effect.