消肿Ⅰ号膏剂的高效液相指纹图谱研究

Study on HPLC fingerprint of Xiaozhong ointment No.1

目的建立消肿I号膏剂量的高效液相指纹图谱。方法采用Waters X-Bridge C18色谱柱(150 mm×4.6 mm,5μm),流动相为乙腈(A)-0.1%磷酸水溶液(B),梯度洗脱(0~18 min、19%A,18~43 min、19%~50%A,43~65 min、50%~19%A),柱温30℃,流速1 mL·min^-1,检测波长210 nm,进样量10μL。结果共指认了10批样品的指纹图谱中的18个共有峰。其中,指标性成分阿魏酸、人参皂苷Rg1、告达庭的相似度较高,重复性好。结论所用方法简便可靠、重复性好,为消肿I号膏剂的进一步研究提供了科学基础。

OBJECTIVE To establish an HPLC fingerprint method for the detection of Xiaozhong ointment No.1.METHODS A Waters X-Bridge column(250 mm×4.6 mm,5μm)was used with acetonitrile as mobile phase A and 0.1%phosphoric acid water solution as mobile phase B.Gradient elution was as follow:0-18 min for 19%A,18-43 min for 19%-50%A,and 43-65 min for 50%-19%A.Column temperature was maintained at 30℃with a flow rate of 1 mL·min^-1.The detection wavelengh was set at 210 nm with the injection volume of 10μL.RESULTS 18 peaks were identified as the common peaks from 10 batches of different samples.The standard components included ferulic acid,ginsenoside Rg1 and caudatin,all of which had high similarity and good reproducibility.CONCLUSION The method is convenient,accurate and easy to perform,providing a scientific basis for further study of Xiaozhong ointment No.1.