大柴胡汤治疗肝郁气滞型胰腺炎功效物质基础与作用机制网络研究

Study on effective components and mechanism of Dachaihu Decoction in treatment of pancreatitis(syndrome of liver depression and qi stagnation)

目的探讨大柴胡汤治疗肝郁气滞型胰腺炎的功效物质基础和作用机制。方法基于网络药理学,首先运用Cytoscape 3.6.0等软件构建疏肝理气中药功效关键靶点蛋白质相互作用(PPI)网络和大柴胡汤治疗胰腺炎"中药方剂-活性成分-关键靶点"多维关系网络,再将2个网络进行交集处理获得大柴胡汤治疗肝郁气滞型胰腺炎"中药方剂-活性成分-关键靶点"功效作用网络,以相关软件进行分析。结果共获得AKT1、VEGFA、PRSS1等59个疏肝理气中药功效关键靶点;共收集到大柴胡汤中槲皮素(quercetin)、山柰酚(kaempferol)、黄芩素(baicalein)等83个主要活性成分,治疗肝郁气滞型胰腺炎涉及PTGS2、DPP4、PRSS1等18个疏肝理气靶点以及细胞外基质降解(extracellular matric disassemble)、白细胞介素-17信号通路(IL-17 signaling pathway)、晚期糖基化终末产物与其受体信号通路(AGE-RAGE signaling pathway in diabetic Com plications)等30条相关生物过程和信号通路。结论大柴胡汤多成分、多靶点是其发挥功效作用的基础,运用网络药理学在证候背景下研究中药复方值得深入思考。

Objective To study the effective components and mechanism of action of Dachaihu Decoction(DCHD)in the treatment of pancreatitis(syndrome of liver depression and qi stagnation).Methods The PPI network and the multi-dimensional relationship network of"Chinese material medica prescription active ingredient key target"of DCHD in the treatment of pancreatitis with stagnation of liver qi.Based on network pharmacology,the software such as Cytoscape 3.6.0 was firstly used to construct the PPI interaction network of key targets in the Chinese material medica with the effect of soothing liver and regulating qi("Shugan Liqi"in Chinese)and DCHD"Chinese material medica prescription-effective components-key target"multi-dimensional network for the treatment of pancreatitis,and then treat the two networks as an intersection to obtain the network of DCHD for the treatment of pancreatitis(syndrome of liver depression and qi stagnation)"Chinese material medica prescription-effective components-key target",and finally analyzed with relevant software.Results A total of 59 key targets for Shugan Liqi were obtained such as AKT1,VEGFA,and PRSS1;A total of 83 main active ingredients such as quercetin,kaempferol and baicalein were collected from DCHD.The treatment of pancreatitis(syndrome of liver depression and qi stagnation)involved 18 targets of Ganyu Qizhi such as PTGS2,DPP4,PRSS1,and 30 related biological processes and signaling pathways such as extracellular matrix degeadation,IL-17 signaling pathway,and AGE-RAGE signaling pathway in diabetic complications.Conclusion The multi-component and multi-target in DCHD are the basis for its efficacy.It is worthwhile to deeply think about the use of network pharmacology in the context of syndrome research.