女儿茶化学成分研究

Chemical constituents from Rhamnus heterophylla

目的研究鼠李科鼠李属植物崖枣树Rhamnus heterophylla枝叶(女儿茶)的化学成分。方法综合运用硅胶柱色谱、MCI柱色谱、Sephadex LH-20凝胶柱色谱等多种色谱分离技术进行分离和纯化,根据化合物的理化性质、核磁共振(NMR)数据进行结构鉴定。并采用HPLC-DAD-ESI-MS/MS技术,负离子模式全扫描,对其化学成分进行快速识别。结果从女儿茶70%乙醇回流提取物中分离得到10个化合物,分别鉴定为顺式-大黄素二蒽酮(1)、反式-大黄素二蒽酮(2)、大黄素-8-O-α-L-鼠李糖苷(3)、6-羟基芦荟大黄素(4)、大黄素(5)、大黄酚(6)、大黄素甲醚(7)、山柰酚(8)、木犀草素(9)、槲皮素(10)。除化合物5、8外,其余化合物均首次从女儿茶中分离得到,其中化合物3为首次从鼠李属植物中分离得到。通过分析获得的质谱数据及质谱裂解规律、参考文献,从女儿茶中初步识别出28个化学成分,包括3个羟基苯甲酸类、8个蒽醌类、17个黄酮类成分。结论对女儿茶乙醇提取物化学成分的分析,为其药效物质基础研究及合理开发利用该植物资源提供参考。

Objective To investigate the chemical constituents from Rhamnus heterophylla.Methods Multiple chromatographic separation techniques including silica gel,Sephadex LH-20 gel and MCI gel were employed to isolate and purify the compounds.Their structures were identified by means of the nuclear magnetic resonance(NMR)and physicochemical properties.The chemical compositions were quickly analyzed by HPLC-DAD-ESI-MS/MS and scanned in negative ions.Results These compounds were isolated and determined as cis-aloeemodin bianthrone(1),trans-aloeemodin bianthrone(2),emodin-8-O-rhamnoside(3),citreorosein(4),emodin(5),chrysophanol(6),physcion(7),kaempferol(8),luteolin(9),and quercetin(10).In addition to compounds 5,8,the other compounds are isolated from R.heterophylla for the first time,and the compound 3 is isolated from the genus Rhamnus for the first time.28 Compounds were speculated via comparing MS data(detected mass and MS2 fragment ions)and coupled with related literature,including three hydroxybenzoic acids,eight anthraquinones and 17 flavonoids.Conclusion The analysis of chemical constituents from R.heterophylla could provide the references for the study on pharmacodynamic material basis and reasonable application and development.