含黄芪方剂组方规律及核心药对“黄芪-当归”分子机制的研究

Compatibility law of Astragali Radix formulae and molecular mechanism of core herbal pair “Astragali Radix-Angelica Sinensis”

目的借助中医传承辅助平台和网络药理学对"黄芪-当归"治疗虚劳的分子机制进行探讨。方法通过中医传承辅助平台挖掘《中药成方制剂》数据库中含黄芪的方剂、配伍组合及其主治疾病;探究含黄芪并治疗虚劳的方剂及配伍组合,明确"黄芪-当归"这一核心配伍药对。采用Batman-TCM、Gene Cards和OMIM等网络药理学技术构建药材-成分-靶点网络,并对靶点进行拓扑属性分析、聚类分析、GO分析和KEGG通路分析。结果《中药成方制剂》数据库中共收录含黄芪方剂459条,涉及中药641味。其中,"黄芪-当归"为主治虚劳的核心药对组合,与古籍记载、临床用药相吻合。网络分析表明该药对主要涉及免疫应答、氧化应激、信号传导等生物过程,通过调节pathways in cancer、PI3K-Akt等信号通路来发挥治疗虚劳的作用。结论揭示了"黄芪-当归"药对治疗虚劳的分子机制,为黄芪及其组方的后续研究提供理论依据及参考。

Objective To screen out the molecular mechanism of core herbal pair"Astragali Radix-Angelica Sinensis"for treating consumptive disease by using the Traditional Chinese Medicine Inheritance Support System(TCMISS)and network pharmacology.Methods The prescriptions and commonly used compatibility combinations containing Astragali Radix,and their main treatment of diseases were excavated from TCMISS database,and the core compatibility of"Astragali Radix-Angelica Sinensis"against consumptive disease was illustrated.The herb-ingredients-targets network was constructed through network pharmacology referring to Batman-TCM,GeneCards,OMIM,and so on.Meanwhile,the topology,cluster analysis,GO,and KEGG pathways analysis of the targets were performed.Results TCMISS database contained totally 459 prescriptions containing Astragali Radix involving 641 herbs."Astragali Radix-Angelica Sinensis"was the core compatibility for the treatment of consumptive disease,which was consistent with ancient record and clinical medication.The results also showed that the process of immune response,oxidative stress,and signal transduction were its mainly molecular mechanism,by adjusting the pathways in cancer,PI3 K-Akt signaling pathway to play its treating consumptive disease effect.Conclusion The paper revealed the molecular mechanism of"Astragali Radix-Angelica Sinensis",and provided theoretical basis and reference for the follow-up study of Astragali Radix and its prescriptions.