基于指纹图谱和多成分含量测定的丹参药材皮部和木部化学成分比较研究

Chemical comparison of Salvia miltiorrhiza phloem and xylem based on fingerprint analysis and quantitative analysis of multi-components

目的基于指纹图谱和多成分含量测定对丹参药材皮部和木部的化学成分进行分析与比较,阐明丹参药材不同部位活性成分的分布规律。方法采用高效液相色谱法对不同批次的丹参皮部和木部样品进行分析,分别建立丹参皮部和木部指纹图谱;在此基础上,对丹参皮部和木部样品中的丹参素、迷迭香酸、紫草酸、丹酚酸B、二氢丹参酮I、隐丹参酮、丹参酮I和丹参酮IIA8个活性成分进行含量测定,并结合聚类分析、主成分分析和正交偏最小二乘判别分析等化学计量学方法对丹参皮部和木部化学成分进行比较。结果建立了丹参药材皮部和木部化学指纹图谱,共标定了10个共有峰;含量测定和化学计量学分析结果表明,丹参皮部和木部样品的化学成分存在差异,其中丹酚酸类成分差异不大,而丹参酮类成分差异较大,丹参皮部样品中丹参酮类成分明显高于丹参木部样品。结论通过指纹图谱结合多成分含量测定方法,明确了丹参药材不同部位活性成分的分布差异,为丹参药材资源的开发和利用提供了科学依据。

Objective To comprehensively compare and evaluate the composition of Salvia miltiorrhiza phloem and xylem samples based on fingerprint analysis and quantitative analysis of multi-components.Methods In the present study,an accurate and reliable fingerprint approach was developed using high performance liquid chromatography for chemical comparison ofphloem and xylem samples of S.miltiorrhiza.Furthermore,eight bioactive compounds including four salvianolic acids and four tanshinones in S.miltiorrhiza phloem and xylem samples were simultaneously quantified.Moreover,chemometrics methods were performed to compare and discriminate the phloem and xylem based on the quantitative data.Results The specific fingerprints of phloem and xylem of S.miltiorrhiza were obtained,and a total of 10 common peaks were marked.The quantitative and chemometrics analysis results indicated the content of chemical components in phloem and xylem samples of S.miltiorrhiza were notably different.Obviously,the content of tanshinones were notably different between phloem and xylem samples.The content of tanshinones were significant higher in phloem compared with xylem in S.miltiorrhiza.Conclusion The fingerprint analysis and quantitative analysis of multi-components could be a well-acceptable strategy for chemical comparison of S.miltiorrhiza phloem and xylem.