基于抗炎活性成分的苗药羊耳菊药材质量控制研究

Study on quality control of Miao medicine Inula cappa based on anti-inflammatory active ingredients

目的基于抗炎活性成分研究苗药羊耳菊药材质量控制方法。方法建立羊耳菊有效组分中代表性、特征性化学成分作为指标成分的多指标定量指纹图谱研究。通过多指标成分定量结合指纹图谱分析评价贵州不同产地羊耳菊的质量。结果建立了35批贵州产羊耳菊的高效液相指纹图谱和多指标含量测定方法;指认了羊耳菊药材液相色谱指纹图谱17个共有峰中的8个,结果显示相似度在0.898~0.997,以8个化学成分作为指标,对其进行含量测定,结果显示绿原酸、新绿原酸、隐绿原酸、异绿原酸B、异绿原酸A、异绿原酸C、洋蓟素、木犀草苷的质量分数分别为0.353~3.765、0.056~0.495、0.086~0.526、0.306~2.526、0.861~7.353、0.729~4.268、0.052~0.424、0.148~1.102 mg/g。结论所建立的基于抗炎活性成分的指纹图谱和多指标含量测定研究方法,灵敏度高、准确度好、稳定可靠,可用于苗药羊耳菊药材的质量控制。

Objective To study the quality control method of Miao medicine Inula cappa based on anti-inflammatory active ingredients.Methods Firstly,a representative and characteristic chemical composition of effective components in I.cappa was established as an indicator component for multi-index quantitative fingerprinting.Then,the quality of I.cappa in different areas of Guizhou Province was evaluated by quantitative analysis of multiple indicators and fingerprint analysis.Results The HPLC fingerprints and multi-index content determination methods of 35 batches of I.cappa were established.Eight of 17 common peaks of the liquid chromatographic fingerprints of I.cappa were identified.The results showed that the similarity was 0.898—0.997,eight components of which were used as indicators to determine the content of chlorogenic acid,neochlorogenic acid,cryptochlorogenic acid,isochlorogenic acid B,isochlorogenic acid A,isochlorogenic acid C,cynarin and luteolin,which were 0.353—3.765,0.056—0.495,0.086—0.526,0.306—2.526,0.861—7.353,0.729—4.268,0.052—0.424,0.148—1.102 mg/g,respectively.Conclusion The fingerprinting and multi-index content determination methods based on anti-inflammatory active ingredients established have high sensitivity,good accuracy,stability and reliability,which can be used for quantitative control of Miao medicine I.cappa.