中华苦荬菜黄酮部位制备工艺及抗氧化活性研究

Study on Preparation Technology and Antioxidant Activity of Flavonoid Fraction from Ixeris Chinensis

目的:优选中华苦荬菜黄酮部位的提取及大孔树脂的纯化工艺,考察中华苦荬菜黄酮部位抗氧化活性。方法:以木犀草苷提取率为评价指标,通过单因素试验及正交实验优选提取工艺。在此基础上,考察不同型号的大孔树脂,优选大孔树脂的纯化工艺。采用清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基试验对黄酮提取物进行抗氧化活性评价。结果:最佳提取工艺:12倍量的70%乙醇回流提取2次,每次1.5 h。最佳纯化工艺:HPD100型大孔吸附树脂,上样浓度0.48 mg·mL^-1,最大上样量5 BV,上样速率2 BV·h^-1,洗脱剂70%的乙醇,洗脱速率2 BV·h^-1,洗脱剂用量5 BV。中华苦荬菜醇提物经大孔树脂纯化后的出膏率为3.79%,纯化物中木犀草苷含量为7.04%。中华苦荬菜黄酮部位对DPPH自由基有较好的清除作用。结论:该制备工艺操作简便易行,适用于大规模生产。中华苦荬菜总黄酮具有良好的抗氧化活性。

Objective:To optimize the processes of extaction and macroporous resin purification of flavonoid fraction in Ixeris chinensis,and to evaluate the antioxidant activity of flavonoid fraction. Methods:The monofactorial observation and the orthogonal test were used to optimize the extraction process,with the Luteoin-7-o-glucoside as the indexes. On this basis,explore different types of macroporous resin,mobile state experiments were conducted to study adsorption capability of the most suitable resin. DPPH free redical-scavenging assay was used to test the antioxidant activity. Result:The optimum extraction process was as follows:12 times mount of 70% ethanol was used to extract for 2 times with 1.5 h each time.The optimum macroprous resin purification process was as follows:macroporous resin HPD100 was used,the concentration of the sample solution was 0.48 mg·mL^-1,the maximum sample was 5 BV,the flowing rate was 2 BV·h^-1,the eluant was 70% ethanol and the flowing rate was 2 BV·h^-1,the eluant volume was 5 BV. The extract yield was 3.79% after macroporous resin purification. The content of the Luteoin-7-o-glucoside was 7.04%. Flavonoid fraction showed a certain DPPH free redical-scavenging activity. Conclusion:This optimum process is easy to operate,which can be applied in large-scale production of the flavonoid fraction. The flavonoid fraction of Ixeris chinensis has acertain antioxidant activity.