摘要
实验设计从富含乳酸菌的样品中分离目标菌,通过多次划线传代,结合形态观察与革兰氏染色,获得纯培养物,接下来通过16sRNA基因序列与使用API 50 CH试剂盒进一步将目标菌鉴定到种.教学实践证明通过开展这一综合性实验,可以培养学生良好专业技能,有助于提高学生综合素质和创新思能力.
The experiment designed to isolate the target bacteria from the samples rich in lactic acid bacteria.Through multiple passages,morphological observation and Gram staining,the pure culture was obtained.Then the target bacteria were further identified by 16sRNA gene sequence and API 50CH kit.Teaching practice has proved that this comprehensive experiment can cultivate students'good professional skills and help improve students'comprehensive quality and innovative thinking ability.
作者
王彦凤
王志钢
苑琳
郝慧芳
王潇
WANG Yanftng;WANG Zhigang;YUAN Lin;HAO Huifang;WANG Xiao(School of Life Sciences,Inner Mongolia University,Hohhot,Inner Mongolia 010070)
出处
《科教导刊》
2019年第31期108-109,共2页
The Guide Of Science & Education
基金
2018年引进高层次人才科研启动金(21400-5185112)
关键词
综合性实验
乳酸菌
分离与鉴定
教学实践
comprehensive experiment
Lactobacillus
isolation and identification
teaching practice
