思州鸡BMP15基因第1外显子多态性及其与蛋品质的关联分析

Polymorphisms of BMP15 Gene Exon 1 and Its Association with Egg Quality in Sizhou Chicken

研究旨在探讨思州鸡骨形态发生蛋白15(BMP15)第1外显子多态性及其与蛋品质的相关性。采用PCR产物直接测序法检测思州鸡BMP 15基因第1外显子测序,将测序结果用DNAStar软件校正;利用Excel 2007统计基因型频率数据,并进行卡方适合性检验;通过SPSS 18.0软件对BMP 15基因不同基因型与思州鸡蛋品质间的关联性进行分析。结果显示,思州鸡BMP 15基因第1外显子存在4个SNPs位点:397C/T、473A/G、603C/T和612C/T,均处于Hardy-Weinberg非平衡状态(P<0.05);397C/T和603C/T位点的多态信息含量(PIC)分别为0.3488和0.3299,属于中度多态(0.25<PIC<0.5),473A/G与612C/T位点的PIC分别为0.1527和0.2075,属于低度多态(PIC<0.25)。关联性分析结果表明,397C/T位点对思州鸡蛋白高度、哈氏单位、蛋黄重均有显著影响(P<0.05);473A/G、603C/T位点对思州鸡蛋品质指标均无显著影响(P>0.05);612C/T位点对思州鸡蛋长径有显著影响(P<0.05),提示BMP 15基因第1外显子对鸡蛋品质中蛋白高度、蛋长径具有较大的遗传效应。结果表明,BMP 15基因可能是影响思州鸡蛋品质的基因之一,可作为思州鸡蛋白高度、蛋长径等蛋品质性状的分子标记。

This study was aimed to explore the polymorphisms of BMP 15 gene exon 1 and its association with egg quality in Sizhou chicken.The direct sequencing of PCR products was adopted to detect the polymorphism of BMP 15 gene exon 1 in Sizhou chicken.After direct sequencing,sequence adjusting were conducted using DNAStar software.Data of genotype frequency was counted by Excel 2007 and analyzed byχ2 test.The association of genotype and egg quality were analyzed with SPSS 18.0.The results showed that BMP 15 gene exon 1 in Sizhou chicken had four SNPs:397C/T,473A/G,603C/T and 612C/T,which were not in Hardy-Weinberg equilibrium(P<0.05);The polymorphic information content(PIC)of 397C/T and 603C/T loci were 0.3488 and 0.3299,respectively,belonging to moderate polymorphism(0.25<PIC<0.5).The PIC of 473A/G and 612C/T loci were 0.1527 and 0.2075,respectively,belonging to low polymorphism(PIC<0.25).The results of correlation analysis showed that the 397C/T locus significantly affected the protein height,Hastelloy unit and egg yolk weight of Sizhou chicken(P<0.05);There were no significant difference of 473A/G and 603C/T loci with egg quality in Sizhou chicken(P>0.05);The 612C/T locus had a significant influence on egg length of Sizhou chicken,respectively(P<0.05).This results suggested that BMP 15 gene exon 1 had a larger genetic effect on the protein height and egg length in Sizhou chicken.BMP 15 gene might be one of the genes affecting the egg quality in Sizhou chicken,and could be used as a molecular marker for egg quality traits such as protein height and egg length and diameter in Sizhou chicken.