制备表面图案化的聚丙烯酰胺-丙烯酸水凝胶

Preparation of the surface patterned polyacrylamide-acrylic hydrogel

背景:聚丙烯酰胺水凝胶具备良好的生物相容性,但力学性能较差,影响了其在生物材料领域的应用。目的:通过微模塑图形化压印制备具有特殊尺寸的聚丙烯酰胺-丙烯酸水凝胶。方法:依次将不同体积的聚丙烯酰胺溶液、丙烯酸与过硫酸铵溶液混合,加入含有微模塑图形化印章的孔板中,制备聚丙烯酰胺-丙烯酸水凝胶:A组聚丙烯酰胺溶液1.4 mL,丙烯酸0.1 mL;B组聚丙烯酰胺溶液1.3 mL,丙烯酸0.2 mL;C组聚丙烯酰胺溶液1.2 mL,丙烯酸0.3 mL;D组聚丙烯酰胺溶液1.1 mL,丙烯酸0.4 mL;E组聚丙烯酰胺溶液1.0 mL,丙烯酸0.5 mL;F组聚丙烯酰胺溶液0.9 mL,丙烯酸0.6 mL。6组过硫酸铵溶液均为50 L。光镜下观察水凝胶的图案化结构,电子万能试验机检测水凝胶的力学性能。结果与结论:光镜显示各组水凝胶表面的条纹清晰可见;丙烯酸的加入有效改良了水凝胶的力学性能,随着丙烯酸比例的不断增加,水凝胶的力学性能逐渐增强。结果表明,聚丙烯酸-丙烯酰胺水凝胶具有良好的力学性能,有望在组织工程损伤修复领域具有良好的应用前景。

BACKGROUND:Polyacrylamide hydrogels have good biocompatibility,but their mechanical properties are poor,which affect their application in the field of biomaterials.OBJECTIVE:To prepare the polyacrylamide-acrylic hydrogels with a particular size by micromolding graphical imprinting.METHODS:Polyacrylamide-acrylic hydrogel was prepared by sequentially mixing different volumes of polyacrylamide solution,acrylic acid and ammonium persulfate solution into an orifice plate containing a micromolded patterned seal.Group A:polyacrylamide solution 1.4 mL,acrylic acid 0.1 mL;group B:polyacrylamide solution 1.3 mL,acrylic acid 0.2 mL;group C:polyacrylamide solution 1.2 mL,acrylic acid 0.3 mL;group D:polyacrylamide solution 1.1 mL,acrylic acid 0.4 mL;group E:polyacrylamide solution 1.0 mL,acrylic acid 0.5 mL;group F:polyacrylamide solution 0.9 mL,acrylic acid 0.6 mL.Six groups of ammonium persulfate solution were all 50μL.The patterned structure of the hydrogel was observed under a light microscope.The mechanical properties of the hydrogel were examined by an electronic universal testing machine.RESULTS AND CONCLUSION:Light microscope showed that the stripes on the surface of each group of hydrogels were clearly visible.The addition of acrylic acid effectively improved the mechanical properties of hydrogels.As the proportion of acrylic acid increased,the mechanical properties of hydrogels gradually increased.These results suggest that polyacrylic acid/acrylamide hydrogel has good mechanical properties and is expected to have good application prospects in the field of tissue engineering damage repair.